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Insulin amyloid pol...
Insulin amyloid polymorphs: implications for iatrogenic cytotoxicity
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- Yuzu, Keisuke (author)
- Ehime Univ, Japan
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- Lindgren, Mikael (author)
- Norwegian Univ Sci & Technol, Norway
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- Nyström, Sofie (author)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Zhang, Jun (author)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Mori, Wakako (author)
- Ehime Univ, Japan
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- Kunitomi, Risako (author)
- Ehime Univ, Japan
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- Nagase, Terumasa (author)
- Tokyo Med Univ, Japan
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- Iwaya, Keiichi (author)
- Kyoundo Hosp, Japan
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- Hammarström, Per (author)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Zako, Tamotsu (author)
- Ehime Univ, Japan
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(creator_code:org_t)
- 2020
- 2020
- English.
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In: RSC Advances. - : ROYAL SOC CHEMISTRY. - 2046-2069. ; 10:62, s. 37721-37727
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https://pubs.rsc.org...
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Abstract
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- Amyloid specific fluorescent probes are becoming an important tool for studies of disease progression and conformational polymorphisms in diseases related to protein misfolding and aggregation such as localized and systemic amyloidosis. Herein, it is demonstrated that using the amyloid specific fluorescent probes pFTAA and benzostyryl capped benzothiadiazole BTD21, structural polymorphisms of insulin amyloids are imaged in localized insulin-derived amyloid aggregates formed at subcutaneous insulin-injection sites in patients with diabetes. It is also found that pFTAA and BTD21 could discriminate structural polymorphisms of insulin amyloids, so called fibrils and filaments, formed in vitro. In addition, it is shown that insulin drug preparations used for treating diabetes formed various types of amyloid aggregates that can be assessed and quantified using pFTAA and BTD21. Interestingly, incubated pFTAA-positive insulin preparation aggregates show cytotoxicity while BTD21-positive aggregates are less toxic. From these observations, a variety of amyloid polymorphic structures with different cytotoxicities formed both in vivo and in vitro by various insulin preparations are proposed.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Yuzu, Keisuke
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Lindgren, Mikael
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Nyström, Sofie
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Zhang, Jun
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Mori, Wakako
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Kunitomi, Risako
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show more...
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Nagase, Terumasa
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Iwaya, Keiichi
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Hammarström, Per
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Zako, Tamotsu
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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RSC Advances
- By the university
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Linköping University