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  • Tran, Thuy,1980-Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten (author)

Real-Time Nanoplasmonic Sensor for IgG Monitoring in Bioproduction

  • Article/chapterEnglish2020

Publisher, publication year, extent ...

  • 2020-10-16
  • MDPI,2020
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:liu-171683
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-171683URI
  • https://doi.org/10.3390/pr8101302DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Funding Agencies|European UnionEuropean Union (EU) [841373]; Swedish Innovation Agency (VINNOVA)Vinnova [2016-04120, 2019-00130]
  • Real-time monitoring of product titers during process development and production of biotherapeutics facilitate implementation of quality-by-design principles and enable rapid bioprocess decision and optimization of the production process. Conventional analytical methods are generally performed offline/at-line and, therefore, are not capable of generating real-time data. In this study, a novel fiber optical nanoplasmonic sensor technology was explored for rapid IgG titer measurements. The sensor combines localized surface plasmon resonance transduction and robust single use Protein A-modified sensor chips, housed in a flexible flow cell, for specific IgG detection. The sensor requires small sample volumes (1-150 mu L) and shows a reproducibility and sensitivity comparable to Protein G high performance liquid chromatography-ultraviolet (HPLC-UV). The dynamic range of the sensor system can be tuned by varying the sample volume, which enables quantification of IgG samples ranging from 0.0015 to 10 mg/mL, without need for sample dilution. The sensor shows limited interference from the sample matrix and negligible unspecific protein binding. IgG titers can be rapidly determined in samples from filtered unpurified Chinese hamster ovary (CHO) cell cultures and show good correlation with enzyme-linked immunosorbent assay (ELISA).

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  • Eskilsson, OlofLinköpings universitet,Biofysik och bioteknik,Tekniska fakulteten(Swepub:liu)oloes23 (author)
  • Mayer, FlorianLinköpings universitet,Biofysik och bioteknik,Tekniska fakulteten(Swepub:liu)n/a (author)
  • Gustavsson, RobertLinköpings universitet,Biofysik och bioteknik,Tekniska fakulteten(Swepub:liu)robgu43 (author)
  • Selegård, RobertLinköpings universitet,Biofysik och bioteknik,Tekniska fakulteten(Swepub:liu)robse34 (author)
  • Lundström, IngemarLinköpings universitet,Sensor- och aktuatorsystem,Tekniska fakulteten(Swepub:liu)inglu57 (author)
  • Mandenius, Carl-FredrikLinköpings universitet,Biofysik och bioteknik,Tekniska fakulteten(Swepub:liu)carma16 (author)
  • Martinsson, ErikArgusEye AB, Spannmalsgatan 55, S-58336 Linkoping, Sweden (author)
  • Aili, DanielLinköpings universitet,Biofysik och bioteknik,Tekniska fakulteten(Swepub:liu)danai11 (author)
  • Linköpings universitetBiofysik och bioteknik (creator_code:org_t)

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  • In:Processes: MDPI8:102227-9717

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