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Quantification of o...
Quantification of ocular surface microcirculation by computer assisted video microscopy and diffuse reflectance spectroscopy
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- Kvernebo, Anne Kari (author)
- Univ Oslo, Norway; Oslo Univ Hosp HF, Norway
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- Miyamoto, Takuma (author)
- Lerner Res Inst, OH 44195 USA
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- Sporast, Ane Hasle (author)
- Oslo Univ Hosp HF, Norway; Univ Oslo, Norway
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- Wikslund, Liv Kristin (author)
- Oslo Univ Hosp HF, Norway; Univ Oslo, Norway
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- Masoy, Svein-Erik (author)
- ODI Med AS, Norway
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- Drolsum, Liv (author)
- Oslo Univ Hosp HF, Norway; Univ Oslo, Norway
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- Moe, Morten Carstens (author)
- Oslo Univ Hosp HF, Norway; Univ Oslo, Norway
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- Salerud, Göran (author)
- Linköpings universitet,Avdelningen för medicinsk teknik,Tekniska fakulteten
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- Fukamachi, Kiyotaka (author)
- Lerner Res Inst, OH 44195 USA
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- Kvernebo, Knut (author)
- Univ Oslo, Norway; Oslo Univ Hosp HF, Norway
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(creator_code:org_t)
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2020
- 2020
- English.
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In: Experimental Eye Research. - : ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD. - 0014-4835 .- 1096-0007. ; 201
- Related links:
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https://liu.diva-por... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
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- In piglets we tested the applicability of digital video microscopy and diffuse reflectance spectroscopy for non-invasive assessments of limbal and bulbar conjunctival microcirculation. A priori we postulated that the metabolic rate is higher in limbal as compared to bulbar conjunctiva, and that this difference is reflected in micro vascular structure or function between the two locations. Two study sites, Oslo University Hospital (OUH), Norway and Cleveland Clinic (CC), USA, used the same video microscopy and spectroscopy techniques to record limbal and bulbar microcirculation in sleeping piglets. Recordings were analyzed with custom-made software to quantify functional capillary density, capillary flow velocity and microvascular oxygen saturation in measuring volumes of approximately 0.1 mm(3). The functional capillary density was higher in limbus than in bulbar conjunctiva at both study sites (OUH: 18.1 +/- 2.9 versus 12.2 +/- 2.9 crossings per mm line, p < 0.01; CC: 11.3 +/- 3.0 versus 7.1 +/- 2.8 crossings per mm line, p < 0.01). Median categorial capillary blood flow velocity was higher in bulbar as compared with limbal recordings (CC: 3 (1-3) versus 1 (0-3), p < 0.01). Conjunctival microvascular oxygen saturation was 88 +/- 5.9% in OUH versus 94 +/- 7.5% in CC piglets. Non-invasive digital video microscopy and diffuse reflectance spectroscopy can be used to obtain data from conjunctival microcirculation in piglets. Limbal conjunctival microcirculation has a larger capacity for oxygen delivery as compared with bulbar conjunctiva.
Subject headings
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Keyword
- August krogh; Conjunctival microcirculation; Capillary flow velocity; Functional capillary density; Limbal microcirculation; Microvascular oxygen saturation; ODIN concept
Publication and Content Type
- ref (subject category)
- art (subject category)
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Kvernebo, Anne K ...
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Miyamoto, Takuma
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Sporast, Ane Has ...
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Wikslund, Liv Kr ...
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Masoy, Svein-Eri ...
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Drolsum, Liv
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Moe, Morten Cars ...
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Salerud, Göran
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Fukamachi, Kiyot ...
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Kvernebo, Knut
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Atom and Molecul ...
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Experimental Eye ...
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Linköping University