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Formation of Suppor...
Formation of Supported Lipid Bilayers Derived from Vesicles of Various Compositional Complexity on Conducting Polymer/Silica Substrates
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- Ulmefors, Hanna (author)
- Division of Nano and Biological Physics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden
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- Nissa, Josefin, 1987- (author)
- Linköpings universitet,Laboratoriet för organisk elektronik,Tekniska fakulteten,Linköping University
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- Pace, Hudson, 1982 (author)
- Division of Nano and Biological Physics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden,Chalmers tekniska högskola,Chalmers University of Technology
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- Wahlsten, Olov, 1989 (author)
- Division of Nano and Biological Physics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden,Chalmers tekniska högskola,Chalmers University of Technology
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- Gunnarsson, Anders, 1981 (author)
- Discovery Sciences, BioPharmaceuticals R&D, AstraZeneca, Mölndal, Sweden,AstraZeneca AB
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- Simon, Daniel T, 1978- (author)
- Linköpings universitet,Laboratoriet för organisk elektronik,Tekniska fakulteten,Linköping University
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- Berggren, Magnus, 1968- (author)
- Linköpings universitet,Laboratoriet för organisk elektronik,Tekniska fakulteten,Linköping University
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- Höök, Fredrik, 1966 (author)
- Division of Nano and Biological Physics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden,Chalmers tekniska högskola,Chalmers University of Technology
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- Gustafsson, Hanna, 1982 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2021-04-30
- 2021
- English.
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In: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 37:18, s. 5494-5505
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Abstract
Subject headings
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- Supported lipid bilayers (SLBs) serve important roles as minimalistic models of cellular membranes in multiple diagnostic and pharmaceutical applications as well as in the strive to gain fundamental insights about their complex biological function. To further expand the utility of SLBs, there is a need to go beyond simple lipid compositions to thereby better mimic the complexity of native cell membranes, while simultaneously retaining their compatibility with a versatile range of analytical platforms. To meet this demand, we have in this work explored SLB formation on PEDOT:PSS/silica nanoparticle composite films and mesoporous silica films, both capable of transporting ions to an underlying conducting PEDOT:PSS film. The SLB formation process was evaluated by using the quartz crystal microbalance with dissipation (QCM-D) monitoring, total internal reflection fluorescence (TIRF) microscopy, and fluorescence recovery after photobleaching (FRAP) for membranes made of pure synthetic lipids with or without the reconstituted membrane protein β-secretase 1 (BACE1) as well as cell-derived native lipid vesicles containing overexpressed BACE1. The mesoporous silica thin film was superior to the PEDOT:PSS/silica nanoparticle composite, providing successful formation of bilayers with high lateral mobility and low defect density even for the most complex native cell membranes.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- Supported lipid bilayer
- PEDOT:PSS
- phospholipid
- conjugated polymer
- conducting polymer
- vesicle fusion
- silica
Publication and Content Type
- ref (subject category)
- art (subject category)
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Ulmefors, Hanna
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Nissa, Josefin, ...
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Pace, Hudson, 19 ...
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Wahlsten, Olov, ...
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Gunnarsson, Ande ...
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Simon, Daniel T, ...
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Berggren, Magnus ...
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Höök, Fredrik, 1 ...
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Gustafsson, Hann ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Physical Chemist ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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Langmuir
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Linköping University
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Chalmers University of Technology