Transplantation of autologous cells and porous gelatin microcarriers to promote wound healing

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Larsson, AlexanderLinköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten,Region Östergötland, Hand- och plastikkirurgiska kliniken US (author)

Transplantation of autologous cells and porous gelatin microcarriers to promote wound healing

Article/chapterEnglish2021

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Elsevier Science Ltd,2021
electronicrdacarrier

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LIBRIS-ID:oai:DiVA.org:liu-176186
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-176186URI
https://doi.org/10.1016/j.burns.2020.08.003DOI

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Language:English
Summary in:English

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

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Funding Agencies|Swedish government [LIO-834921]; county councils, the ALF-agreement [LIO-834921]
Definitive treatment to achieve wound healing in major burns frequently include skin transplantation, where split-thickness skin grafts is considered gold standard. This method is associated with several drawbacks. To overcome these hurdles, efforts have been made to develop tissue engineered skin substitutes, often comprised of a combination of cells and biomaterials. In the present study, we aimed to investigate transplantation of autologous keratinocytes and fibroblasts seeded on porous gelatin microcarriers using a porcine wound model. Pre-seeded microcarriers were transplanted to a total of 168 surgical full-thickness wounds (2 cm diameter) on eight adult female pigs and covered with occlusive dressings. The experimental groups included wounds transplanted with microcarriers seeded with the combination of keratinocytes and fibroblasts, microcarriers seeded with each cell type individually, microcarriers without cells, each cell type in suspension, and NaCl control. Wounds were allowed to heal for one, two, four or eight weeks before being excised and fixated for subsequent histological and immunohistochemical analysis. In vitro, we confirmed that viable cells populate the surface and the pores of the microcarriers. In vivo, the microcarriers were to a large extent degraded after two weeks. After one week, all treatment groups, with the exception of microcarriers alone, displayed significantly thicker neo-epidermis compared to controls. After two weeks, wounds transplanted with microcarriers seeded with cells displayed significantly thicker neo-epidermis compared to controls. After four weeks there was no difference in the thickness of neo-epidermis. In conclusion, the experiments performed illustrate that autologous cells seeded on porous gelatin microcarriers stimulates the re-epithelialization of wounds. This method could be a promising candidate for skin transplantation. Future studies will focus on additional outcome parameters to evaluate long-term quality of healing following transplantation. (c) 2020 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
Burns; Wound healing; Transplantation; Tissue engineering; Guided tissue regeneration

Added entries (persons, corporate bodies, meetings, titles ...)

Briheim, KristinaLinköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten(Swepub:liu)kribr96 (author)
Hanna, VictorLinköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten(Swepub:liu)n/a (author)
Gustafsson, KarinLinköpings universitet,Institutionen för fysik, kemi och biologi,Tekniska högskolan(Swepub:liu)kargu22 (author)
Starkenberg, AnnikaLinköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten(Swepub:liu)annst28 (author)
Vintertun, HansLinköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten,Region Östergötland, Hand- och plastikkirurgiska kliniken US(Swepub:liu)hanjo30 (author)
Kratz, GunnarLinköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten,Region Östergötland, Hand- och plastikkirurgiska kliniken US(Swepub:liu)gunkr37 (author)
Junker, JohanLinköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten,Region Östergötland, Katastrofmedicinskt centrum(Swepub:liu)johju92 (author)
Linköpings universitetAvdelningen för kirurgi, ortopedi och onkologi (creator_code:org_t)

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In:Burns: Elsevier Science Ltd47:3, s. 601-6100305-41791879-1409

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