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mRNA expression of ...
mRNA expression of oxidative-reductive proteins in boars with documented different fertility can identify relevant prognostic biomarkers
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- Alvarez-Rodriguez, Manuel (author)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Univ Autonoma Barcelona, Spain
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- Martinez Serrano, Cristina (author)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten
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- Roca, Jordi (author)
- Univ Murcia, Spain
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- Rodriguez-Martinez, Heriberto (author)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten
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(creator_code:org_t)
- Elsevier Science Ltd, 2021
- 2021
- English.
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In: Research in Veterinary Science. - : Elsevier Science Ltd. - 0034-5288 .- 1532-2661. ; 141, s. 195-202
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https://doi.org/10.1...
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Abstract
Subject headings
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- Oxidative stress unbalance is a major factor causing impairment of sperm function and, ultimately, sperm death. In this study, we identified transcriptomic and proteomic markers for oxidative-related protectors from the generation of reactive oxygen species (ROS) in spermatozoa from breeding boars with documented high- or lowfertility. Particular attention was paid to glutathione peroxidases, and to transcripts related to DNA stabilization and compaction, as protamine and transition proteins. mRNA cargo analysis was performed using porcinespecific micro-arrays (GeneChip (R) miRNA 4.0 and GeneChip (R) Porcine Gene 1.0 ST) and qPCR validation. Differences between fertility-classed boars were ample among biomarkers; some upregulated only at protein level (catalase (CAT), superoxide dismutase 1 (SOD1) and glutathione proteins), or only at the mRNA level (ATOX1, Antioxidant Protein 1). In addition, protamines 2 and 3, essential for sperm DNA condensation and also transition proteins 1 and 2 (TNP1 and TNP2), required during histone-to-protamine replacement, were overexpressed in spermatozoa from high-fertile boars. This up-regulation seems concerted to reduce DNA accessibility to ROS attack, protecting the DNA. The upregulated intracellular phospholipid hydroperoxide glutathione peroxidase (GPx4), in high-fertile boars at mRNA level, can be considered a most relevant biomarker for fertility disclosure during sperm evaluation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
Keyword
- ROS; Oxidation; Fertility; AI; Spermatozoa; Pig
Publication and Content Type
- ref (subject category)
- art (subject category)
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