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A hexasaccharide from capsular polysaccharide of carbapenem-resistant Klebsiella pneumoniae KN2 is a ligand of Toll-like receptor 4

Lee, I-Ming (author)
Acad Sinica, Taiwan
Huang, Tzu-Yin (author)
Acad Sinica, Taiwan; Natl Sun Yat Sen Univ, Taiwan
Yang, Feng-Ling (author)
Acad Sinica, Taiwan
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Johansson, Victor (author)
Linköpings universitet,Tekniska fakulteten,Biologi
Hsu, Chun-Ru (author)
Natl Taiwan Ocean Univ, Taiwan
Hsieh, Pei-Fang (author)
Natl Taiwan Univ, Taiwan
Chen, Shin-Tai (author)
Acad Sinica, Taiwan
Yang, Yen-Ju (author)
Acad Sinica, Taiwan
Konradsson, Peter, 1957- (author)
Linköpings universitet,Kemi,Tekniska fakulteten
Sheu, Jyh-Horng (author)
Natl Sun Yat Sen Univ, Taiwan; Natl Sun Yat Sen Univ, Taiwan
Wang, Jin-Town (author)
Natl Taiwan Univ, Taiwan; Natl Taiwan Univ Hosp, Taiwan
Wu, Shih-Hsiung (author)
Acad Sinica, Taiwan; Natl Sun Yat Sen Univ, Taiwan
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 (creator_code:org_t)
ELSEVIER SCI LTD, 2022
2022
English.
In: Carbohydrate Polymers. - : ELSEVIER SCI LTD. - 0144-8617 .- 1879-1344. ; 278
Related links:
https://urn.kb.se/re...
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https://doi.org/10.1...
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Klebsiella pneumoniae serotype KN2 is a carbapenem-resistant strain and leads to the health care-associated in-fections, such as bloodstream infections. Its capsular polysaccharide (CPS) was isolated and cleaved by a specific enzyme from a bacteriophage into a hexasaccharide-repeating unit. With GC-MS, NMR, and Mass analyses, the structure of KN2 CPS was determined to be {-> 3)-beta-D-Glcp-(1 -> 3)-[alpha-D-GlcpA-(1 -> 4)-beta-D-Glcp-(1 -> 6)]-alpha-D-Galp- (1 -> 6)-beta-D-Galp-(1 -> 3)-beta-D-Galp-(1 ->}(n). We demonstrated that 1 mu g/mL CPS could stimulate J774A.1 murine macrophages to release tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) in vitro. Also, we proved that KN2 CPS induced the immune response through Toll-like receptor 4 (TLR4) in the human embryonic kidney (HEK)-293 cells. Strikingly, the hexasaccharide alone shows the same immune response as the CPS, suggesting that the hexasaccharide can shape the adaptive immunity to be a potential vaccine adjuvant. The glucuronic acid (GlcA) on other polysaccharides can affect the immune response, but the GlcA-reduced KN2 CPS and hex-asaccharide still maintain their immunomodulatory activities.

Subject headings

NATURVETENSKAP  -- Kemi -- Organisk kemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences -- Organic Chemistry (hsv//eng)

Keyword

K. pneumoniae; Capsular polysaccharide; Bacteriophage glycosidase; Toll-like receptor 4

Publication and Content Type

ref (subject category)
art (subject category)

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