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GBS-MeDIP: A protoc...
GBS-MeDIP: A protocol for parallel identification of genetic and epigenetic variation in the same reduced fraction of genomes across individuals
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- Rezaei, Shiva (author)
- Linköpings universitet,Biologi,Tekniska fakulteten,University of Tabriz, Tabriz, Iran
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- Uffenorde, Julia (author)
- Linköpings universitet,Avdelningen för neurobiologi,Medicinska fakulteten,Region Östergötland, Kirurgiska kliniken US
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- Gimm, Oliver, 1967- (author)
- Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten,Region Östergötland, Kirurgiska kliniken US
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- Hosseinpour Feizi, Mohammad Ali (author)
- University of Tabriz, Tabriz, Iran
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- Miemczyk, Stefan (author)
- Linköpings universitet,Biologi,Tekniska fakulteten
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- Coutinho, Luiz Lehmann (author)
- Uppsala University, Sweden
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- Jensen, Per, 1956- (author)
- Linköpings universitet,Biologi,Tekniska fakulteten
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- Guerrero-Bosagna, Carlos, 1975- (author)
- Linköpings universitet,Biologi,Tekniska fakulteten,Uppsala University, Sweden
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- Pértille, Fábio (author)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,Biologi,Tekniska fakulteten,Uppsala University, Sweden; College of Agriculture (ESALQ), Brazil
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(creator_code:org_t)
- Cell Press, 2022
- 2022
- English.
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In: STAR Protocols. - : Cell Press. - 2666-1667. ; 3:1
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https://liu.diva-por... (primary) (Raw object)
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Abstract
Subject headings
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- Summary:The GBS-MeDIP protocol combines two previously described techniques, Genotype-by-Sequencing (GBS) and Methylated-DNA-Immunoprecipitation (MeDIP). Our method allows for parallel and cost-efficient interrogation of genetic and methylomic variants in the DNA of many reduced genomes, taking advantage of the barcoding of DNA samples performed in the GBS and the subsequent creation of DNA pools, then used as an input for the MeDIP. The GBS-MeDIP is particularly suitable to identify genetic and methylomic biomarkers when resources for whole genome interrogation are lacking.
Subject headings
- NATURVETENSKAP -- Data- och informationsvetenskap (hsv//swe)
- NATURAL SCIENCES -- Computer and Information Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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