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Overexpression of a...
Overexpression of a soybean Globin (GmGlb1-1) gene reduces plant susceptibility to Meloidogyne incognita
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- Basso, Marcos Fernando (author)
- W5 Norte, Brazil; Natl Inst Sci & Technol, Brazil
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- Lourenco-Tessutti, Isabela Tristan (author)
- W5 Norte, Brazil; Natl Inst Sci & Technol, Brazil
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- Moreira-Pinto, Clidia Eduarda (author)
- W5 Norte, Brazil; Natl Inst Sci & Technol, Brazil; Fed Univ Brasilia, Brazil
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- Mendes, Reneida Aparecida Godinho (author)
- W5 Norte, Brazil; Fed Univ Brasilia, Brazil
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- Paes-de-Melo, Bruno (author)
- W5 Norte, Brazil; Natl Inst Sci & Technol, Brazil
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- das Neves, Maysa Rosa (author)
- W5 Norte, Brazil
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- Macedo, Amanda Ferreira (author)
- Univ Sao Paulo, Brazil
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- Figueiredo, Viviane (author)
- Fed Univ Rio Janeiro, Brazil
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- Grandis, Adriana (author)
- Univ Sao Paulo, Brazil
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- Macedo, Leonardo Lima Pepino (author)
- W5 Norte, Brazil; Natl Inst Sci & Technol, Brazil
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- Arraes, Fabricio Barbosa Monteiro (author)
- W5 Norte, Brazil; Natl Inst Sci & Technol, Brazil
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- Costa, Marcos Mota do Carmo (author)
- W5 Norte, Brazil
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- Togawa, Roberto Coiti (author)
- W5 Norte, Brazil; Natl Inst Sci & Technol, Brazil
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- Enrich Prast, Alex (author)
- Linköpings universitet,Tema Miljöförändring,Filosofiska fakulteten,Biogas Solutions Research Center,Fed Univ Rio Janeiro, Brazil
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- Marcelino-Guimaraes, Francismar Correa (author)
- Natl Inst Sci & Technol, Brazil; Embrapa Soybean, Brazil
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- Gomes, Ana Cristina Meneses Mendes (author)
- W5 Norte, Brazil
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- Silva, Maria Cristina Mattar (author)
- W5 Norte, Brazil; Natl Inst Sci & Technol, Brazil
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- Floh, Eny Iochevet Segal (author)
- Univ Sao Paulo, Brazil
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- Buckeridge, Marcos Silveira (author)
- Univ Sao Paulo, Brazil
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- Engler, Janice de Almeida (author)
- Natl Inst Sci & Technol, Brazil; Univ Cote dAzur, France
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- Grossi-de-Sa, Maria Fatima (author)
- W5 Norte, Brazil; Natl Inst Sci & Technol, Brazil; Univ Catolica Brasilia, Brazil
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(creator_code:org_t)
- 2022-09-16
- 2022
- English.
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In: Planta. - : SPRINGER. - 0032-0935 .- 1432-2048. ; 256:4
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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Abstract
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- Non-symbiotic globin class #1 (Glb1) genes are expressed in different plant organs, have a high affinity for oxygen, and are related to nitric oxide (NO) turnover. Previous studies showed that soybean Glb1 genes are upregulated in soybean plants under flooding conditions. Herein, the GmGlb1-1 gene was identified in soybean as being upregulated in the nematode-resistant genotype PI595099 compared to the nematode-susceptible cultivar BRS133 during plant parasitism by Meloidogyne incognita. The Arabidopsis thaliana and Nicotiana tabacum transgenic lines overexpressing the GmGlb1-1 gene showed reduced susceptibility to M. incognita. Consistently, gall morphology data indicated that pJ2 nematodes that infected the transgenic lines showed developmental alterations and delayed parasitism progress. Although no significant changes in biomass and seed yield were detected, the transgenic lines showed an elongated, etiolation-like growth under well-irrigation, and also developed more axillary roots under flooding conditions. In addition, transgenic lines showed upregulation of some important genes involved in plant defense response to oxidative stress. In agreement, higher hydrogen peroxide accumulation and reduced activity of reactive oxygen species (ROS) detoxification enzymes were also observed in these transgenic lines. Thus, based on our data and previous studies, it was hypothesized that constitutive overexpression of the GmGlb1-1 gene can interfere in the dynamics of ROS production and NO scavenging, enhancing the acquired systemic acclimation to biotic and abiotic stresses, and improving the cellular homeostasis. Therefore, these collective data suggest that ectopic or nematode-induced overexpression, or enhanced expression of the GmGlb1-1 gene using CRISPR/dCas9 offers great potential for application in commercial soybean cultivars aiming to reduce plant susceptibility to M. incognita.
Subject headings
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
Keyword
- Glycine max; Glyma.11G121800; New biotechnology tools; Phytoglobins; Plant-nematode interaction; Root-knot nematodes; PI595099; BRS133
Publication and Content Type
- ref (subject category)
- art (subject category)
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Basso, Marcos Fe ...
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Lourenco-Tessutt ...
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Moreira-Pinto, C ...
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Mendes, Reneida ...
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Paes-de-Melo, Br ...
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das Neves, Maysa ...
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Macedo, Amanda F ...
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Figueiredo, Vivi ...
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Grandis, Adriana
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Macedo, Leonardo ...
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Arraes, Fabricio ...
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Costa, Marcos Mo ...
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Togawa, Roberto ...
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Enrich Prast, Al ...
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Marcelino-Guimar ...
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Gomes, Ana Crist ...
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Silva, Maria Cri ...
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Floh, Eny Iochev ...
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Buckeridge, Marc ...
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Engler, Janice d ...
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Grossi-de-Sa, Ma ...
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- About the subject
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Agricultural Bio ...
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and Plant Biotechnol ...
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Planta
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Linköping University