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Human Stem Cell-Der...
Human Stem Cell-Derived TRPV1-Positive Sensory Neurons : A New Tool to Study Mechanisms of Sensitization
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- Schrenk-Siemens, Katrin (author)
- Heidelberg Univ, Germany
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- Pohle, Jörg (author)
- Heidelberg Univ, Germany; Grunenthal GmbH, Germany
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- Rostock, Charlotte (author)
- Heidelberg Univ, Germany
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- Abd El Hay, Muad (author)
- Heidelberg Univ, Germany; Ernst Strungmann Inst, Germany
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- Lam, Ruby M. (author)
- NIH, MD 20892 USA
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- Szczot, Marcin (author)
- Linköpings universitet,Centrum för social och affektiv neurovetenskap,Medicinska fakulteten,NIH, MD 20892 USA
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- Lu, Shiying (author)
- Heidelberg Univ, Germany; Oliver Wyman GmbH, Germany
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- Chesler, Alexander T. (author)
- NIH, MD 20892 USA
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- Siemens, Jan (author)
- Heidelberg Univ, Germany
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(creator_code:org_t)
- 2022-09-17
- 2022
- English.
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In: Cells. - : MDPI. - 2073-4409. ; 11:18
- Related links:
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
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- Somatosensation, the detection and transduction of external and internal stimuli such as temperature or mechanical force, is vital to sustaining our bodily integrity. But still, some of the mechanisms of distinct stimuli detection and transduction are not entirely understood, especially when noxious perception turns into chronic pain. Over the past decade major progress has increased our understanding in areas such as mechanotransduction or sensory neuron classification. However, it is in particular the access to human pluripotent stem cells and the possibility of generating and studying human sensory neurons that has enriched the somatosensory research field. Based on our previous work, we describe here the generation of human stem cell-derived nociceptor-like cells. We show that by varying the differentiation strategy, we can produce different nociceptive subpopulations with different responsiveness to nociceptive stimuli such as capsaicin. Functional as well as deep sequencing analysis demonstrated that one protocol in particular allowed the generation of a mechano-nociceptive sensory neuron population, homogeneously expressing TRPV1. Accordingly, we find the cells to homogenously respond to capsaicin, to become sensitized upon inflammatory stimuli, and to respond to temperature stimulation. The efficient and homogenous generation of these neurons make them an ideal translational tool to study mechanisms of sensitization, also in the context of chronic pain.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Annan klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Other Clinical Medicine (hsv//eng)
Keyword
- somatosensation; human pluripotent stem cells; nociceptor-like cells; homogenous neuronal population; TRPV1 responders; translational tool
Publication and Content Type
- ref (subject category)
- art (subject category)
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