A Novel High Discriminatory Protocol for the Detection of Borrelia afzelii, Borrelia burgdorferi Sensu Stricto and Borrelia garinii in Ticks

Funding Agencies|PRIN_MIUR2012 [2012A4F828]; European Union [38-2-7-19]; Swedish Research Council (Vetenskapsradet, VR) [2018-03830]
Bacteria of the Borrelia burgdorferi sensu lato complex are the causative agents of Lyme borreliosis (LB). Even if the conventional diagnosis of LB does not rely on the species itself, an accurate species identification within the complex will provide a deepened epidemiological scenario, a better diagnosis leading to a more targeted therapeutic approach, as well as promote the general publics awareness. A comparative genomics approach based on the 210 Borrelia spp. genomes available in 2019 were used to set up three species-specific PCR protocols, able to detect and provide species typing of Borrelia afzelii, Borrelia burgdorferi sensu stricto (s.s.) and Borrelia garinii, the three most common and important human pathogenic Lyme Borrelia species in Europe. The species-specificity of these protocols was confirmed on previously identified B. afzelii, B. burgdorferi s.s. and B. garinii specimens detected in Ixodes ricinus samples. In addition, the protocols were validated on 120 DNA samples from ticks collected in Sweden, showing 88% accuracy, 100% precision, 72% sensitivity and 100% specificity. The proposed approach represents an innovative tool in epidemiological studies focused on B. burgdorferi s.l. occurrence in ticks, and future studies could suggest its helpfulness in routine diagnostic tests for health care.

Perini, MatteoUniv Milan, Italy (author)
Cafiso, AlessandraUniv Milan, Italy (author)
Nodari, RiccardoUniv Milan, Italy (author)
Wilhelmsson, PeterLinköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Dept Clin Microbiol, Sweden(Swepub:liu)petwi60 (author)
Lindgren, Per-EricLinköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Dept Clin Microbiol, Sweden(Swepub:liu)perli15 (author)
Omazic, AnnaNatl Vet Inst, Sweden (author)
Ullman, KarinNatl Vet Inst, Sweden (author)
Moutailler, SaraEcole Natl Vet Alfort, France (author)
Kjellander, PetterSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för ekologi,Department of Ecology,Swedish Univ Agr Sci, Sweden(Swepub:slu)50066 (author)
Bazzocchi, ChiaraUniv Milan, Italy (author)
Grandi, GiulioSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,National Veterinary Institute SVA,Natl Vet Inst, Sweden; Swedish Univ Agr Sci, Sweden (author)
Univ Milan, ItalyAvdelningen för inflammation och infektion (creator_code:org_t)
Sveriges lantbruksuniversitet

By the author/editor: Chiappa, Giulia; Perini, Matteo; Cafiso, Alessand ...; Nodari, Riccardo; Wilhelmsson, Pet ...; Lindgren, Per-Er ...; show more...; Omazic, Anna; Ullman, Karin; Moutailler, Sara; Kjellander, Pett ...; Bazzocchi, Chiar ...; Grandi, Giulio; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Infectious Medic ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Microbiology

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biological Syste ...

By the university: Linköping University; Swedish University of Agricultural Sciences

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