Dietary Sugar Shifts Mitochondrial Metabolism and Small RNA Biogenesis in Sperm

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Ramesh, Rashmi,1984-Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten (author)

Dietary Sugar Shifts Mitochondrial Metabolism and Small RNA Biogenesis in Sperm

Article/chapterEnglish2023

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2023-02-23
MARY ANN LIEBERT, INC,2023
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LIBRIS-ID:oai:DiVA.org:liu-192683
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-192683URI
https://doi.org/10.1089/ars.2022.0049DOI

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Language:English
Summary in:English

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Subject category:art swepub-publicationtype

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Funding Agencies|Swedish Research Council [2015-03141]; Ragnar Soderbergs foundation; Knut and Alice Wallenberg foundation [2015.0165]
Aims: Increasing concentrations of dietary sugar results in a linear accumulation of triglycerides in male Drosophila, while inducing a U-shaped obesity response in their offspring. Here, using a combination of proteomics and small RNA (sRNA) sequencing, we aimed at understanding the molecular underpinning in sperm for such plasticity.Results: Proteomic analysis of seminal vesicles revealed that increasing concentrations of dietary sugar resulted in a bell-shaped induction of proteins involved in metabolic/redox regulation. Using stains and in vivo redox reporter flies, this pattern could be explained by changes in sperm production of reactive oxygen species (ROS), more exactly mitochondria-derived H2O2. By quenching ROS with the antioxidant N-acetyl cysteine and performing sRNA-seq on sperm, we found that sperm miRNA is increased in response to ROS. Moreover, we found sperm mitosRNA to be increased in high-sugar diet conditions (independent of ROS). Reanalyzing our previously published data revealed a similar global upregulation of human sperm mitosRNA in response to a high-sugar diet, suggesting evolutionary conserved mechanisms.Innovation: This work highlights a fast response to dietary sugar in mitochondria-produced H2O2 in Drosophila sperm and identifies redox-sensitive miRNA downstream of this event.Conclusions: Our data support a model where changes in the sperm mitochondria in response to dietary sugar are the primary event, and changes in redox homoeostasis are secondary to mitochondrial ROS production. These data provide multiple candidates for paternal intergenerational metabolic responses as well as potential biomarkers for human male fertility.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Farmaceutiska vetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Pharmaceutical Sciences hsv//eng
diet; sperm; proteomics; small RNA; mitochondrial ROS; mitochondrial small RNA; miR-10; tsRNA

Added entries (persons, corporate bodies, meetings, titles ...)

Skog, SigneLinköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten(Swepub:liu)sigsk47 (author)
Örkenby, Lovisa,1992-Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten(Swepub:liu)lovor74 (author)
Örtegren (Kugelberg), Unn,1975-Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten(Swepub:liu)unnor29 (author)
Nätt, Daniel,1980-Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten(Swepub:liu)danis31 (author)
Öst, Anita,1965-Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten(Swepub:liu)anios27 (author)
Linköpings universitetAvdelningen för cellbiologi (creator_code:org_t)

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In:Antioxidants and Redox Signaling: MARY ANN LIEBERT, INC38:16-18, s. 1167-11831523-08641557-7716

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