Search: onr:"swepub:oai:DiVA.org:liu-201131" >
RhoB expression ass...
RhoB expression associated with chemotherapy response and prognosis in colorectal cancer
-
- Kopsida, Maria (author)
- Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten
-
- Liu, Na (author)
- Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
-
- Kotti, Angeliki (author)
- Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten
-
show more...
-
- Wang, Jing (author)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,Science for Life Laboratory
-
- Jensen, Lasse D, 1979- (author)
- Linköpings universitet,Avdelningen för diagnostik och specialistmedicin,Medicinska fakulteten,Region Östergötland, Klinisk farmakologi
-
- Jothimani, Ganesan (author)
- Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu, India
-
- Hildesjö, Camilla, 1969- (author)
- Linköpings universitet,Medicinska fakulteten,Avdelningen för kirurgi, ortopedi och onkologi,Region Östergötland, Regionalt cancercentrum
-
- Haapaniemi, Staffan (author)
- Linköpings universitet,Medicinska fakulteten,Avdelningen för kirurgi, ortopedi och onkologi,Region Östergötland, Kirurgiska kliniken ViN
-
- Zhong, Wen (author)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
-
- Pathak, Surajit, 1977- (author)
- Linköpings universitet,Medicinska fakulteten,Avdelningen för kirurgi, ortopedi och onkologi,Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu, India
-
- Sun, Xiao-Feng, 1959- (author)
- Linköpings universitet,Medicinska fakulteten,Avdelningen för kirurgi, ortopedi och onkologi,Region Östergötland, Onkologiska kliniken US
-
show less...
-
(creator_code:org_t)
- BioMed Central (BMC), 2024
- 2024
- English.
-
In: Cancer Cell International. - : BioMed Central (BMC). - 1475-2867. ; 24:1
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://liu.diva-por... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Purpose: To examine the role of RhoB expression in relation to chemotherapy response, clinical outcomes and associated signaling pathways in colorectal cancer patients.Materials and methods: The study included 5 colon cancer cell lines, zebrafish embryos and 260 colorectal cancer patients treated with 5-fluorouracil (5-FU) and oxaliplatin (OXL). The methods consisted of CRISPR/Cas9, reactive oxygen species (ROS), caspase-3 activity, autophagy flux, in-silico RNA sequencing and immunohistochemistry. Gene expression analysis and pathway analysis were conducted using RNA-seq data.Results: All cancer lines tested, including SW480, SW480-KO13 (RhoB knockout), SW480-KO55 (RhoB knockout), HCT116 and HCT116-OE (RhoB overexpressed), exhibited cytotoxicity to 5-FU and OXL. RhoB knockout cell lines demonstrated significantly reduced migration compared to the control cell lines. Furthermore, RhoB played a role in caspase-3-dependent apoptosis, regulation of ROS production and autophagic flux. The mRNA sequencing data indicated lower expression levels of oncogenes in RhoB knockout cell lines. The zebrafish model bearing SW480-KO showed a light trend toward tumor regression. RhoB expression by immunohistochemistry in patients was increased from normal mucosa to tumor samples. In patients who received chemotherapy, high RhoB expression was related to worse survival compared to low RhoB expression. Furthermore, the molecular docking analysis revealed that OXL had a higher binding affinity for RhoB than 5-FU, with a binding affinity of -7.8 kcal/mol and HADDOCK predicted molecular interactions between RhoB and caspase 3 protein. Gene-set enrichment analysis supported these findings, showing that enrichment of DNA damage response pathway and p53 signaling in RhoB overexpression treatment group, while the RhoB knockout treatment group exhibited enrichment in the negative regulation pathway of cell migration.Conclusion: RhoB was negatively associated with chemotherapy response and survival in colorectal cancers. Therefore, RhoB inhibition may enhance chemotherapeutic responses and patient survival.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- RhoB; Colorectal cancer; 5-fluorouracil; Oxaliplatin; Survival; Signaling pathway
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Kopsida, Maria
-
Liu, Na
-
Kotti, Angeliki
-
Wang, Jing
-
Jensen, Lasse D, ...
-
Jothimani, Ganes ...
-
show more...
-
Hildesjö, Camill ...
-
Haapaniemi, Staf ...
-
Zhong, Wen
-
Pathak, Surajit, ...
-
Sun, Xiao-Feng, ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Cancer and Oncol ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
- Articles in the publication
-
Cancer Cell Inte ...
- By the university
-
Linköping University