onr:"swepub:oai:DiVA.org:liu-25224"
Search: onr:"swepub:oai:DiVA.org:liu-25224" > A Lectin Immunosens...
- 1 of 1
- Previous record
- Next record
- To hitlist
A Lectin Immunosensor Technique for Determination of α1-Acid Glycoprotein Fucosylation
-
- Liljeblad, Mathias (author)
- Linköpings universitet,Klinisk kemi,Hälsouniversitetet
-
- Rydén, Ingvar (author)
- Department of Clinical Chemistry, Kalmar County Hospital, Kalmar, Sweden
-
- Ohlson, Sten (author)
- Division of Biochemistry/Biotechnology, Department of Chemistry and Biomedical Sciences, University of Kalmar, Kalmar, Sweden
- show more...
- show less...
- (creator_code:org_t)
- Elsevier BV, 2001
- 2001
- English.
- In: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 288:2, s. 216-224
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- The fucosylation of α1-acid glycoprotein (AGP), an acute-phase protein, is known to change in association with inflammatory diseases. Thus, fucosylation of AGP could be a potential diagnostic or prognostic marker. The change in fucosylation has previously been investigated using crossed affinoimmunoelectrophoresis, high-pH anion-exchange chromatography, and lectin ELISA. This study describes a surface plasmon resonance-based affinity biosensor assay for quantification of the fucosylation of AGP. Diluted EDTA plasma or serum was injected directly in a BIACORE 2000 biosensor. AGP was captured on the sensor surface using immobilized antibodies and a fucose-binding lectin from Aleuria aurentia was then used for the detection of fucosylation. The feature of the biosensor makes it possible to determine both the amount of bound AGP and the amount of bound lectin. Using a calibration curve it was possible to obtain a fucosylation ratio that was independent of AGP concentration. The assay was validated against a lectin ELISA and used to follow inflammation in patients with severe burns.
Keyword
- a1-acid glycoprotein
- Aleuria aurentia lectin
- Fucose
- Glycosylation
- Surface plasmon resonance
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Analytical Biochemistry (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Liljeblad, Mathi ...
- Rydén, Ingvar
- Ohlson, Sten
- Lundblad, Arne, ...
- Påhlsson, Peter, ...
- Articles in the publication
- Analytical Bioch ...
- By the university
- Linköping University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
