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Leucocyte activatio...
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Bengtsson, Torbjörn,1955-Linköpings universitet,Hälsouniversitetet,Medicinsk mikrobiologi
(author)
Leucocyte activation by collagen-stimulated platelets in whole blood
- Article/chapterEnglish2002
Publisher, publication year, extent ...
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2009-07-08
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Informa UK Limited,2002
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:liu-26382
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-26382URI
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https://doi.org/10.1080/00365510260390000DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Interaction between vascular cells plays an important role in the initial phases of the inflammatory process, but the mechanisms responsible for cell-cell communication are not fully understood. In this study, activation of leucocytes and platelets in heparinized whole blood was assessed using lumi-aggregometry. This technique enables simultaneous measurement of aggregation and oxygen radical production by monitoring impedance and luminol-amplified chemiluminescence (CL), respectively. Collagen induced aggregation and CL, depending on dose, and markedly enhanced subsequent aggregation and CL-response triggered by the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe). Collagen stimulation of whole blood down- and upregulated the expression of L-selectin and CD11b, respectively. Monoclonal antibodies against sialyl LewisX and P-selectin caused a pronounced inhibition of the oxidative burst, triggered by collagen itself or by a combination of collagen and fMet-Leu-Phe. Furthermore, the Arg-Gly-Asp-Ser(RGDS)-peptide effectively inhibited collagentriggered aggregation and CL, and the subsequent enhancement of the fMet-Leu-Phe-induced responses. This suggests that fibrinogen plays a part in linking platelet GpIIb/IIIa with CD11b on the leucocyte surface. However, neither anti-CD11b nor the PI-peptide (containing the ?-chain motif in fibrinogen that interacts with CD11b) counteracted the stimulatory effects of activated platelets on leucocyte functions. The selectin- and integrin-antagonizing substances were ineffective on the CL-responses induced by fMet-Leu-Phe itself. This study suggests that, through selectin- and integrin-dependent interaction, activated platelets potentiate leucocyte aggregation and oxygen radical production, which might be important for the outcome of inflammatory reactions.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Grenegård, Magnus,1963-Linköpings universitet,Hälsouniversitetet,Farmakologi(Swepub:liu)maggr06
(author)
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Grenegård, M
(author)
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Linköpings universitetHälsouniversitetet
(creator_code:org_t)
Related titles
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In:Scandinavian Journal of Clinical and Laboratory Investigation: Informa UK Limited62:6, s. 451-4620036-55131502-7686
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