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- Bengtsson, Torbjörn,1955-Linköpings universitet,Hälsouniversitetet,Medicinsk mikrobiologi (author)
Leucocyte activation by collagen-stimulated platelets in whole blood
- Article/chapterEnglish2002
Publisher, publication year, extent ...
- 2009-07-08
- Informa UK Limited,2002
- printrdacarrier
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- LIBRIS-ID:oai:DiVA.org:liu-26382
- https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-26382URI
- https://doi.org/10.1080/00365510260390000DOI
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- Language:English
- Summary in:English
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- Interaction between vascular cells plays an important role in the initial phases of the inflammatory process, but the mechanisms responsible for cell-cell communication are not fully understood. In this study, activation of leucocytes and platelets in heparinized whole blood was assessed using lumi-aggregometry. This technique enables simultaneous measurement of aggregation and oxygen radical production by monitoring impedance and luminol-amplified chemiluminescence (CL), respectively. Collagen induced aggregation and CL, depending on dose, and markedly enhanced subsequent aggregation and CL-response triggered by the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe). Collagen stimulation of whole blood down- and upregulated the expression of L-selectin and CD11b, respectively. Monoclonal antibodies against sialyl LewisX and P-selectin caused a pronounced inhibition of the oxidative burst, triggered by collagen itself or by a combination of collagen and fMet-Leu-Phe. Furthermore, the Arg-Gly-Asp-Ser(RGDS)-peptide effectively inhibited collagentriggered aggregation and CL, and the subsequent enhancement of the fMet-Leu-Phe-induced responses. This suggests that fibrinogen plays a part in linking platelet GpIIb/IIIa with CD11b on the leucocyte surface. However, neither anti-CD11b nor the PI-peptide (containing the ?-chain motif in fibrinogen that interacts with CD11b) counteracted the stimulatory effects of activated platelets on leucocyte functions. The selectin- and integrin-antagonizing substances were ineffective on the CL-responses induced by fMet-Leu-Phe itself. This study suggests that, through selectin- and integrin-dependent interaction, activated platelets potentiate leucocyte aggregation and oxygen radical production, which might be important for the outcome of inflammatory reactions.
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- MEDICINE
- MEDICIN
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- Grenegård, Magnus,1963-Linköpings universitet,Hälsouniversitetet,Farmakologi(Swepub:liu)maggr06 (author)
- Grenegård, M (author)
- Linköpings universitetHälsouniversitetet (creator_code:org_t)
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- In:Scandinavian Journal of Clinical and Laboratory Investigation: Informa UK Limited62:6, s. 451-4620036-55131502-7686
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