Search: onr:"swepub:oai:DiVA.org:liu-26458" >
Comparison of serol...
Comparison of serologic and genetic porB-based typing of Neisseria gonorrhoeae : consequences for future characterization
-
- Unemo, Magnus, 1970- (author)
- National Reference Laboratory for Pathogenic Neisseria, Örebro Medical Centre Hospital, Örebro, Sweden
-
- Olcén, Per, 1943- (author)
- National Reference Laboratory for Pathogenic Neisseria, Örebro Medical Centre Hospital, Örebro, Sweden
-
- Albert, Jan (author)
- Karolinska Institutet
-
show more...
-
- Fredlund, Hans, 1952- (author)
- National Reference Laboratory for Pathogenic Neisseria and Unit for Infectious Disease Control, Department of Clinical Microbiology, Örebro University Hospital, Örebro
-
show less...
-
(creator_code:org_t)
- 2003
- 2003
- English.
-
In: Journal of Clinical Microbiology. - 0095-1137 .- 1098-660X. ; 41:9, s. 4141-4147
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Due to temporal changes in the epidemiology of gonorrhea, a precise characterization of Neisseria gonorrhoeae is essential. In the present study genetic heterogeneity in the porB genes of N. gonorrhoeae was examined, and serovar determination was compared to porB gene sequencing. Among 108 N. gonorrhoeae isolates, phylogenetic analysis of the entire porB alleles (924 to 993 bp) identified 87 unique sequences. By analyzing only the four to six most heterogeneous porB gene regions (174 to 363 bp), 86 out of these 87 genetic variants were identified. Consequently, analysis of shorter highly variable regions of the porB gene generates high-level discriminatory ability as well as fast, objective, reproducible, and portable data for epidemiological characterization of N. gonorrhoeae. Regarding putative antigenic epitopes of PorB for Genetic Systems monoclonal antibodies (MAbs), some of the previous findings were confirmed, but new findings were also observed. For several of the MAbs, however, the precise amino acid residues of PorB critical for single-MAb reactivity were difficult to identify. In addition, repeated serovar determination of 108 N. gonorrhoeae isolates revealed discrepancies for 34 isolates, mostly due to nonreproducible reactivity with single MAbs. Thus, the prospects of a genetic typing system with congruent translation of the serovar determination seem to be limited. In conclusion, analysis of short highly variable regions of the porB gene could form the basis for a fast molecular epidemiological tool for the examination of emergence and transmission of N. gonorrhoeae strains within the community.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database