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Effect of heat dena...
Effect of heat denaturation on beta-lactoglobulin-induced gastrointestinal sensitization in rats : denatured betaLG induces a more intensive local immunologic response than native betaLG
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Rytkonen, J (författare)
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Karttunen, TJ (författare)
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Karttunen, R (författare)
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Valkonen, KH (författare)
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- Jenmalm, Maria, 1971- (författare)
- Linköpings universitet,Hälsouniversitetet,Pediatrik
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Alatossava, T (författare)
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- Björkstén, B (författare)
- Karolinska Institutet
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Kokkonen, J (författare)
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(creator_code:org_t)
- 2008-09-22
- 2002
- Engelska.
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Ingår i: Pediatric Allergy and Immunology. - : Wiley. - 0905-6157 .- 1399-3038. ; 13:4, s. 269-277
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Beta-lactoglobulin (▀LG) is one of the first foreign antigens encountered by a newborn child, and it is the major allergen causing cow's milk allergy. Heat denaturation causes changes to the protein structure, but the significance of heat-induced changes for immunogenicity or allergenicity is not known. To clarify how heat denaturation affects allergenicity and immunogenicity, we immunized Hooded-Lister rat pups with intra-peritoneal injections of native or heat-denatured ▀LG at days 43 and 62 after birth. The animals were then fed native and denatured milk products twice weekly from 73 to 101 days of age with a feeding tube, after which they were allowed cheese and milk ad libitum, until they were killed on day 131. Total immunoglobulin (Ig)E and ▀LG-specific IgG1 and IgG2a levels were determined from serum samples. Spontaneous interleukin-4 (IL-4) and interferon-? (IFN-?) production was measured from duodenal specimens, and specimens of gastrointestinal mucosae were studied for the presence of inflammatory cells. The rats immunized with native ▀LG had higher levels of total serum IgE than the unimmunized controls or the rats immunized with heat-denatured ▀LG, while heat-denatured ▀LG induced a significantly more intensive mononuclear inflammatory cell and eosinophil infiltration in the gastroduodenal mucosa. The ▀LG -specific IgG antibody and IL-4 and IFN-? responses were similar in the two groups of immunized animals. Hence, denaturation modifies the immunogenic and allergenic properties of ▀LG. Heat-denatured ▀LG induces a more intensive local reaction in the gastrointestinal mucosa, while there is some evidence for enhanced systemic allergic sensitization by native ▀LG. ⌐ 2002 Blackwell Munksgaard.
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- MEDICIN
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