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Inhibition of cathe...
Inhibition of cathepsin D prevents free-radical-induced apoptosis in rat cardiomyocytes
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- Öllinger, Karin, 1962- (author)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Patologi,Klinisk patologi och klinisk genetik
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(creator_code:org_t)
- Elsevier BV, 2000
- 2000
- English.
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In: Archives of Biochemistry and Biophysics. - : Elsevier BV. - 0003-9861 .- 1096-0384. ; 373:2, s. 346-351
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Apoptosis was inhibited in rat cardiomyocytes pretreated with the aspartic protease inhibitor pepstatin A and subsequently exposed to naphthazarin (5,8-dihydroxy-1,4-naphthoquinone). Cathepsin D was released from lysosomes to the cytosol upon exposure to naphthazarin, and the enzyme activity decreased simultaneously. Later, cathepsin D reappeared in granules of increased size, and enzyme activity was restored. Activation of caspase-3- like proteases was detected, and the number of cells showing apoptotic morphology increased with time. Pepstatin A pretreatment did not prevent release of cathepsin D from lysosomes but did significantly inhibit subsequent naphthazarin-induced caspase activation and apoptotic morphology. This suggests that cathepsin D exerts its apoptosis-stimulating effect upstream of caspase-3-like activation. (C) 2000 Academic Press.
Keyword
- MEDICINE
- MEDICIN
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- ref (subject category)
- art (subject category)
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