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An LXXLL motif in n...
An LXXLL motif in nuclear receptor corepressor mediates ligand-induced repression of the thyroid stimulating hormone-β gene
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- Loinder, Kristina, 1963- (author)
- Linköpings universitet,Cellbiologi,Hälsouniversitetet
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- Söderström, Mats, 1958- (author)
- Linköpings universitet,Cellbiologi,Hälsouniversitetet
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(creator_code:org_t)
- Elsevier BV, 2005
- 2005
- English.
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In: Journal of Steroid Biochemistry and Molecular Biology. - : Elsevier BV. - 0960-0760 .- 1879-1220. ; 97:4, s. 322-327
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Nuclear receptor corepressor (N-CoR) regulates gene expression through interaction with DNA-bound nuclear receptors, recruiting multicomponent repressor complexes to the sites of target genes. We recently reported the presence of an LXXLL motif in N-CoR, and showed that this motif interacts in vitro and in vivo with retinoic acid receptor α (RARα) and thyroid hormone receptor β (TRβ). Transient transfection experiments now suggest that TRβ and N-CoR act synergistically and may both be required for ligand-induced repression from the negative TR response element in the thyroid stimulating hormone-β (TSHβ) gene promoter. Mutation of the LXXLL motif in N-CoR abolished ligand-induced repression at this response element. Furthermore, in vitro binding of N-CoR to a complex between TRβ and the negative TR response element was strictly ligand-dependent. We conclude that N-CoR and TRβ cooperate in the regulation of the TSHβ gene and that the ligand-dependent repression is mediated by the LXXLL motif in N-CoR.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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