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Interlaboratory Com...
Interlaboratory Comparison of PCR-Based Identification and Genogrouping of Neisseria meningitidis
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- Taha, Muhamed-Kheir (author)
- Neisseria Unit and the French National Reference Center for Meningococci, Institut Pasteur, Paris, France
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- Alonso, Jean-Michel (author)
- Neisseria Unit and the French National Reference Center for Meningococci, Institut Pasteur, Paris, France
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- Cafferkey, Mary (author)
- Irish Meningococcal and Meningitis Reference Laboratory, The Children's University Hospital, Dublin, Ireland
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- Caugant, Dominique A. (author)
- WHO Collaborating Center for Reference and Research on Meningococci, Division of Infectious Disease Control, Norwegian Institute of Public Health and Institute of Oral Biology, University of Oslo, Oslo, Norway
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- Clarke, Stuart C. (author)
- Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital and Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow
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- Diggle, Mathew A. (author)
- Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital, Glasgow
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- Fox, Andrew (author)
- Meningococcal Reference Unit, Health Protection Agency, Manchester Royal Infirmary, Manchester, United Kingdom
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- Frosch, Matthias (author)
- Institute for Hygiene and Microbiology, National Reference Center for Meningococci, University of Würzburg, Germany
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- Gray, Stephen J. (author)
- Meningococcal Reference Unit, Health Protection Agency, Manchester Royal Infirmary, Manchester, United Kingdom
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- Guiver, Malcolm (author)
- Meningococcal Reference Unit, Health Protection Agency, Manchester Royal Infirmary, Manchester, United Kingdom
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- Heuberger, Sigrid (author)
- National Reference Centre for Meningococci, Austrian Agency for Health and Food Safety, Graz, Austria
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- Kalmusova, Jitka (author)
- National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, Prague, Czech Republic
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- Kesanopoulos, Konstantinos (author)
- National Meningococcal Reference Laboratory, NIPH, Athens, Greece
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- Klem, Anne-Marie (author)
- WHO Collaborating Center for Reference and Research on Meningococci, Division of Infectious Disease Control, Norwegian Institute of Public Health, Oslo, Norway
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- Kriz, Paula (author)
- National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, Prague, Czech Republic
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- Marsh, John (author)
- Meningococcal Reference Unit, Health Protection Agency, Manchester Royal Infirmary, Manchester, United Kingdom
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- Mölling, Paula (author)
- National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, University Hospital, Örebro, Sweden
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- Murphy, Karen (author)
- Irish Meningococcal and Meningitis Reference Laboratory, The Children's University Hospital, Dublin, Ireland
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- Olcén, Per, 1943- (author)
- National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, University Hospital, Örebro, Sweden
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- Sanou, Oumar (author)
- Centre Muraz, Bobo Dioulasso, Burkina Faso
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- Tzanakaki, Georgina (author)
- National Meningococcal Reference Laboratory, NIPH, Athens, Greece
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- Vogel, Ulrich (author)
- Institute for Hygiene and Microbiology, National Reference Center for Meningococci, University of Würzburg, Germany
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(creator_code:org_t)
- 2005
- 2005
- English.
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In: Journal of Clinical Microbiology. - 0095-1137 .- 1098-660X. ; 43:1, s. 144-149
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes (crgA, ctrA, 16S rRNA, and porA). The PCR-based nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7%, respectively. Most of the laboratories also performed genogrouping assays (siaD and mynB/sacC). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.
Keyword
- MEDICINE
- MEDICIN
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- ref (subject category)
- art (subject category)
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- By the author/editor
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Taha, Muhamed-Kh ...
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Alonso, Jean-Mic ...
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Cafferkey, Mary
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Caugant, Dominiq ...
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Clarke, Stuart C ...
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Diggle, Mathew A ...
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show more...
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Fox, Andrew
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Frosch, Matthias
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Gray, Stephen J.
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Guiver, Malcolm
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Heuberger, Sigri ...
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Kalmusova, Jitka
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Kesanopoulos, Ko ...
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Klem, Anne-Marie
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Kriz, Paula
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Marsh, John
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Mölling, Paula
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Murphy, Karen
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Olcén, Per, 1943 ...
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Sanou, Oumar
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Tzanakaki, Georg ...
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Vogel, Ulrich
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Journal of Clini ...
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Linköping University