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Organelle transport...
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Immerstrand, Charlotte,1974-Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
(author)
Organelle transport in melanophores analyzed by white light image correlation spectroscopy
- Article/chapterEnglish2007
Publisher, publication year, extent ...
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Wiley,2007
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:liu-38154
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-38154URI
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https://doi.org/10.1111/j.1365-2818.2007.01743.xDOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Intracellular transport of organelles, vesicles and proteins is crucial in all eukaryotic cells, and is accomplished by motor proteins that move along cytoskeletal filaments. A widely used model of intracellular transport is Xenopus laevis melanophores. These cells help the frog to change color by redistributing melanin-containing organelles in the cytoplasm. The high contrast of the pigment organelles permits changes in distribution to be observed by ordinary light microscopy; other intracellular transport systems often require fluorescence labeling. Here we have developed white light Image Correlation Spectroscopy (ICS) to monitor aggregation and dispersion of pigment. Hitherto in ICS, images of fluorescent particles from Confocal Laser Scanning Microscopy (CLSM) have been used to calculate autocorrelation functions from which the density can be obtained. In the present study we show that ICS can be modified to enable analysis of light-microscopy images; it can be used to monitor pigment aggregation and dispersion, and distinguish between different stimuli. This new approach makes ICS applicable not only to fluorescent but also to black-and-white images from light or electron microscopy, and is thus very versatile in different studies of movement of particles on the membrane or in the cytoplasm of cells without potentially harmful fluorescence labeling and activation.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Hedlund, Joel,1978-Linköpings universitet,Bioinformatik,Tekniska högskolan(Swepub:liu)joehe05
(author)
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Magnusson, Karl-Eric,1946-Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet(Swepub:liu)karma55
(author)
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Sundqvist, Tommy,1949-Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet(Swepub:liu)tomsu30
(author)
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Holmgren-Peterson, Kajsa,1964-Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet(Swepub:liu)kajho23
(author)
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Linköpings universitetMedicinsk mikrobiologi
(creator_code:org_t)
Related titles
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In:Journal of Microscopy: Wiley225:3, s. 275-2820022-27201365-2818
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