Search: onr:"swepub:oai:DiVA.org:liu-41920" >
Protein microarray ...
Protein microarray analysis as a tool for monitoring cellular autoreactivity in type 1 diabetes patients and their relatives
-
Vrabelova, Z (author)
-
Kolouskova, S (author)
-
Böhmova, K (author)
-
show more...
-
- Faresjö, Maria, 1971- (author)
- Linköpings universitet,Hälsouniversitetet,Pediatrik,Hälsouniversitetet i Linköping
-
Sumnik, Z (author)
-
Pechova, M (author)
-
Kverka, M (author)
-
Chudoba, D (author)
-
Zacharovova, K (author)
-
Stadlerova, G (author)
-
Pithova, P (author)
-
Hladikova, M (author)
-
Stechova, K (author)
-
show less...
-
(creator_code:org_t)
- Hindawi Limited, 2007
- 2007
- English.
-
In: Pediatric Diabetes. - : Hindawi Limited. - 1399-543X .- 1399-5448. ; 8:5, s. 252-260
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Background: Autoreactive T cells have a crucial role in type 1 diabetes (T1D) pathogenesis.: Objectives: The aim of our study was to monitor the in vitro production of cytokines by peripheral blood mononuclear cells (PBMCs) after stimulation with diabetogenic autoantigens. Subjects: Ten T1D patients (tested at the time of diagnosis and 6 and 12 months later), 10 first-degree relatives of the T1D patients, and 10 controls underwent the study. Methods: PBMCs were stimulated with glutamic acid decarboxylase 65 (GAD65) amino acids (a.a.) 247-279, 509-528, and 524-543, proinsulin a.a. 9-23, and tyrosine phosphatase (islet antigen-2)/R2 a.a. 853-872. Interleukin (IL)-2, IL-4, IL-5, IL-6, IL-10, IL-13, interferon (IFN)-γ, tumor necrosis factor β, transforming growth factor β1, and granulocyte colony-stimulating factor (GCSF) were analyzed by protein microarray. Results: Differences in cytokine(s) poststimulatory and mainly in basal production were observed in all groups. The most prominent findings were in controls, the higher basal levels of IL-2, IL-4, IL-5, IL-13, and GCSF were observed when compared with relatives (p < 0.05, for all). After stimulation in controls, there was a significant decrease in IL-2, IL-13, GCSF, and IFN-γ (p < 0.05, for all). The group of relatives was the most variable in poststimulatory production. A strong correlation between cytokines production was found but groups differed in this aspect. Conclusion: By multiplex analysis, it may be possible, for example, to define the risk immunological response pattern among relatives or to monitor the immune response in patients on immune modulation therapy. © 2007 The Authors Journal compilation © 2007 Blackwell Munksgaard.
Keyword
- autoantigen
- autoreactive T cells
- cytokine
- protein microaray
- T1D
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Vrabelova, Z
-
Kolouskova, S
-
Böhmova, K
-
Faresjö, Maria, ...
-
Sumnik, Z
-
Pechova, M
-
show more...
-
Kverka, M
-
Chudoba, D
-
Zacharovova, K
-
Stadlerova, G
-
Pithova, P
-
Hladikova, M
-
Stechova, K
-
show less...
- Articles in the publication
-
Pediatric Diabet ...
- By the university
-
Linköping University
-
Jönköping University