Search: onr:"swepub:oai:DiVA.org:liu-43367" >
Increased uptake of...
-
Keita, Åsa,1973-Linköpings universitet,Hälsouniversitetet,Institutionen för klinisk och experimentell medicin
(author)
Increased uptake of non-pathogenic E. coli via the follicle-associated epithelium in longstanding ileal Crohn's disease
- Article/chapterEnglish2008
Publisher, publication year, extent ...
-
2008
-
Wiley,2008
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:liu-43367
-
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-43367URI
-
https://doi.org/10.1002/path.2337DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
In Crohn's disease (CD), inflammation is driven by luminal commensal micro-organisms, however, mechanisms of early phases of inflammation need further clarification. The earliest observable lesions of recurrent CD are microscopic erosions at the specialized follicle-associated epithelium (FAE), which lines the Peyer's patches. Therefore, our aim was to investigate the mucosal barrier to non-pathogenic bacteria in FAE of CD. The FAE of macroscopically normal ileum from patients with longstanding CD, ulcerative colitis, and controls was studied in Ussing chambers regarding electrophysiology and permeability to 51Cr-EDTA, horseradish peroxidase, and non-pathogenic E. coli strains. Transepithelial passage routes and uptake into dendritic cells were studied by confocal and electron microscopy. FAE of CD showed increased numbers of adherent bacteria, after E. coli exposure in Ussing chambers, as well as spontaneously in non-exposed archival surgical tissues. Further, we found increased uptake of fluorescent E. coli K-12 and HB101 across FAE of CD, but not in ulcerative colitis. Microscopy demonstrated intercellular and transcellular uptake of E. coli in CD, but only transcellular in controls. FAE exposed to E. coli demonstrated changes in conductance and 51Cr-EDTA permeability, suggesting that bacteria affected the paracellular pathway in CD mucosa. Following bacterial uptake, CD mucosa also demonstrated an increased percentage of E. coli co-localizing with dendritic cells, and augmented tissue release of TNF-α. Our data present novel insights into the pathophysiology of CD by demonstrating a previously unrecognized defect of FAE barrier to bacteria in ileal CD, leading to increased load of commensal bacteria to the inductive sites of mucosal immunity. Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Subject headings and genre
-
Adult Aged Bacterial Adhesion *Bacterial Translocation Case-Control Studies Colitis
-
Ulcerative/immunology/microbiology Crohn Disease/genetics/immunology/*microbiology Dendritic Cells/microbiology Escherichia coli/*physiology Female Humans *Ileum Immunoen
-
MEDICINE
-
MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
-
Salim, Sa´ad,1977-Linköpings universitet,Hälsouniversitetet,Kirurgi(Swepub:liu)saasa68
(author)
-
Jiang, T.Department of Clinical and Experimental Medicine Linköping University
(author)
-
Yang, P-CIntestinal Disease Research Program McMaster University, Hamilton, Canada
(author)
-
Franzén, LennartAleris Medilab Täby
(author)
-
Söderkvist, Peter,1953-Linköpings universitet,Hälsouniversitetet,Cellbiologi(Swepub:liu)petso43
(author)
-
Magnusson, Karl-Eric,1946-Linköpings universitet,Hälsouniversitetet,Medicinsk mikrobiologi(Swepub:liu)karma55
(author)
-
Söderholm, Johan D,1958-Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Kirurgi,Kirurgiska kliniken i Östergötland med verksamhet i Linköping, Norrköping och Motala(Swepub:liu)sodda63
(author)
-
Linköpings universitetHälsouniversitetet
(creator_code:org_t)
Related titles
-
In:Journal of Pathology: Wiley215:2, s. 135-1440022-34171096-9896
Internet link
Find in a library
To the university's database
- By the author/editor
-
Keita, Åsa, 1973 ...
-
Salim, Sa´ad, 19 ...
-
Jiang, T.
-
Yang, P-C
-
Franzén, Lennart
-
Söderkvist, Pete ...
-
show more...
-
Magnusson, Karl- ...
-
Söderholm, Johan ...
-
show less...
- Articles in the publication
-
Journal of Patho ...
- By the university
-
Linköping University