Polychromatic fingerprinting of excitation emission matrices

Gatto, E. (author): Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy

Malik, Muhammad Ali (author): Linköpings universitet,Tekniska högskolan,Institutionen för fysik, kemi och biologi

Di, Natale C. (author): Di Natale, C., Department of Electronic Engineering, University of Rome Tor Vergata, Via del Politecnico 1, 00133 Rome, Italy

Paolesse, R. (author): Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy

D'Amico, A. (author): Department of Electronic Engineering, University of Rome Tor Vergata, Via del Politecnico 1, 00133 Rome, Italy

Lundström, Ingemar (author): Linköpings universitet,Tekniska högskolan,Tillämpad Fysik

Filippini, Daniel (author): Linköpings universitet,Tekniska högskolan,Tillämpad Fysik

(creator_code:org_t)

Wiley, 2008
2008
English.
In: Chemistry - A European Journal. - : Wiley. - 0947-6539 .- 1521-3765. ; 14:20, s. 6057-6060

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

The ability of a ubiquitous polychromatic excitation to support the fingerprinting of the Excitation Emission Matrices (EEM) of fluorescent indicators was reported. Solutions of fluorescent molecules, with concentration of 2µM for the absorption and 10µM for the emission measurements were prepared. Spectroscopic grade THF and distilled water passed through Milli-Q purification system were used as solvents. EEM measurements were carried out on a spectrofluorimeter, operating in the 390-700 nm detection range, with excitations at 20 nm intervals within the same range. During the measurement, the screen displayed a sequence of 50 colors and for every color, the transmission and emission spectrum of both the reference and the sample were recorded. Illuminating colors in the red region are not able to excite fluorescence and this can be seen in the spectra for red light.

By the author/editor: Gatto, E.; Malik, Muhammad ...; Di, Natale C.; Paolesse, R.; D'Amico, A.; Lundström, Ingem ...; show more...; Filippini, Danie ...; show less...

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