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G-protein interacti...
G-protein interactions with receptor-derived peptides chemisorbed on gold
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- Petoral Jr., Rodrigo M. (author)
- Linköpings universitet,Tillämpad Fysik,Tekniska högskolan
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- Herland, Anna (author)
- Linköpings universitet,Tillämpad Fysik,Tekniska högskolan
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- Broo, Kerstin (author)
- Linköpings universitet,Tillämpad Fysik,Tekniska högskolan
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- Uvdal, Kajsa (author)
- Linköpings universitet,Tillämpad Fysik,Tekniska högskolan
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(creator_code:org_t)
- 2003-10-10
- 2003
- English.
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In: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 19:24, s. 10304-10309
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Interactions between the functional bovine brain G-protein and receptor-derived peptidea chemically adsorbed on gold surfaces are studied. The peptides are designed to mimic the third ic-loop (aa 361-373) of the Alpha 2a-adrenergic receptor (α 2AR). These segments are linked to a surface using the thiol-gold chemistry, and the protein interaction studies are conducted to investigate the key function of recognition. The chemical composition and binding strength of the peptide monolayers onto a gold surface are characterized using X-ray photoelectron spectroscopy and infrared (IR) spectroscopy. Strong molecular binding of the adsorbates to the gold surface is attained, and the presence of amide-related IR vibrations verified the composition of the peptides. Bovine brain G-protein adsorption studies on these molecular monolayers are performed using the surface plasmon resonance technique. The arginine-rich peptide, which is a direct mimicry of the receptor, has a higher affinity for G-protein than the lysine-rich and alanine-rich derived peptides, showing that arginine residue has special importance for the G-protein interaction with the receptor.
Keyword
- TECHNOLOGY
- TEKNIKVETENSKAP
Publication and Content Type
- ref (subject category)
- art (subject category)
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