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Effects of CNI-1493...
Effects of CNI-1493 on human granulocyte functions
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- Abdalla, Hana (author)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Forslund, Tony (author)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Schön, Thomas (author)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Stendahl, Olle (author)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Sundqvist, Tommy (author)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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(creator_code:org_t)
- Elsevier BV, 2006
- 2006
- English.
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In: Immunobiology. - : Elsevier BV. - 0171-2985 .- 1878-3279. ; 211:3, s. 191-197
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- During acute bacterial infections such as sepsis and meningitis, activation of inflammatory mediators such as nitric oxide (NO) plays a crucial role in both pathogenesis and host defense. We have previously reported that CNI-1493, a macrophage deactivator, reduced mortality in infant rats infected with Haemophilus influenzae type b (Hib) with associated decrease in the number of granulocytes in the infected tissue. The aim of the present study was to investigate how CNI-1493 affects granulocytes and macrophages in vitro. Murine macrophages (RAW 264.7) pre-incubated with CNI-1493 prior to activation with lipopolysaccharide (LPS)/interferon gamma (IFNγ) had decreased NO production measured as NO2−/NO3− levels and reduction in inducible NO-synthase (iNOS) expression. Reactive oxygen species (ROS) production was increased in formylmethionyl-leucyl-phenylalanine (FMLP)-stimulated granulocytes following CNI-1493 treatment, whereas F-actin content, motility and chemotaxis were decreased under the same conditions. The effects of CNI-1493 on both NO production in LPS/IFNγ-activated macrophages and ROS production, F-actin content, motility and chemotaxis in granulocytes, may contribute to the reduced inflammatory response and increased survival in Hib-infected animals treated with CNI-1493.
Keyword
- CNI-1493
- Granulocytes
- iNOS
- Macrophages
- ROS
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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