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  • Flegel, Willy AUniversity Hospital, Ulm (author)

D variants at the RhD vestibule in the weak D type 4 and Eurasian D clusters

  • Article/chapterEnglish2009

Publisher, publication year, extent ...

  • Wiley,2009
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:liu-53044
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-53044URI
  • https://doi.org/10.1111/j.1537-2995.2009.02102.xDOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • BACKGROUND: One branch of the RHD phylogenetic tree is represented by the weak D type 4 cluster of alleles with F223V as the primordial amino acid substitution. F223V as well as a large number of further substitutions causing D variants are located at the extracellular RhD protein vestibule, which represents the entrance to the transmembraneous channel of the RhD protein. STUDY DESIGN AND METHODS: RHD and RHCE nucleotide sequences were determined from genomic DNA and cDNA. D epitope patterns were established with commercial monoclonal anti-D panels. RESULTS: The RHD alleles DOL-1 and DOL-2 had the two amino acid substitutions M170T (509T>C) and F223V (667T>G) in common. DOL-2 harbored the additional substitution L378V (1132C>G). Both alleles were observed in Africans and are probably evolutionary related. DMI carried M170I (510G>A), which differed from the DOL-typical substitution. DFW and DFL harbored the substitutions H166P (497A>C) and Y165C (494A>G). The antigen densities of DOL-1, DFL, and DFW were only moderately reduced. CONCLUSION: DOL-1 and DOL-2 belong to the weak D type 4 cluster of RHD alleles. Together with DMI, DFL, and DFW they represent D variants with amino acid substitutions located at extracellular loops 3 or 4 lining the RhD protein vestibule. These substitutions were of minor influence on antigen density while adjacent substitutions in the transmembraneous section caused weak D antigen expression. All these D variants were partial D and alloanti-D immunizations have been observed in DOL-1, DMI, and DFL carriers. The substitution at position 170 causes partial D although located deep in the vestibule.

Subject headings and genre

  • MEDICINE
  • MEDICIN

Added entries (persons, corporate bodies, meetings, titles ...)

  • von Zabern, Inge (author)
  • Doescher, Andrea (author)
  • Wagner, Franz F (author)
  • Strathmann, Klaus P (author)
  • Geisen, Christof (author)
  • Plafi, MiodragÖstergötlands Läns Landsting,Linköpings universitet,Transfusionsmedicin,Hälsouniversitetet,Klinisk immunologi och transfusionsmedicin,Landstinget i Östergötland (author)
  • Pisacka, Martin (author)
  • Poole, Joyce (author)
  • Polin, Helene (author)
  • Gabriel, Christian (author)
  • Avent, Neil D (author)
  • University Hospital, UlmTransfusionsmedicin (creator_code:org_t)

Related titles

  • In:Transfusion: Wiley49:6, s. 1059-10690041-11321537-2995

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