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D variants at the R...
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Flegel, Willy AUniversity Hospital, Ulm
(author)
D variants at the RhD vestibule in the weak D type 4 and Eurasian D clusters
- Article/chapterEnglish2009
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Wiley,2009
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printrdacarrier
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LIBRIS-ID:oai:DiVA.org:liu-53044
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-53044URI
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https://doi.org/10.1111/j.1537-2995.2009.02102.xDOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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BACKGROUND: One branch of the RHD phylogenetic tree is represented by the weak D type 4 cluster of alleles with F223V as the primordial amino acid substitution. F223V as well as a large number of further substitutions causing D variants are located at the extracellular RhD protein vestibule, which represents the entrance to the transmembraneous channel of the RhD protein. STUDY DESIGN AND METHODS: RHD and RHCE nucleotide sequences were determined from genomic DNA and cDNA. D epitope patterns were established with commercial monoclonal anti-D panels. RESULTS: The RHD alleles DOL-1 and DOL-2 had the two amino acid substitutions M170T (509T>C) and F223V (667T>G) in common. DOL-2 harbored the additional substitution L378V (1132C>G). Both alleles were observed in Africans and are probably evolutionary related. DMI carried M170I (510G>A), which differed from the DOL-typical substitution. DFW and DFL harbored the substitutions H166P (497A>C) and Y165C (494A>G). The antigen densities of DOL-1, DFL, and DFW were only moderately reduced. CONCLUSION: DOL-1 and DOL-2 belong to the weak D type 4 cluster of RHD alleles. Together with DMI, DFL, and DFW they represent D variants with amino acid substitutions located at extracellular loops 3 or 4 lining the RhD protein vestibule. These substitutions were of minor influence on antigen density while adjacent substitutions in the transmembraneous section caused weak D antigen expression. All these D variants were partial D and alloanti-D immunizations have been observed in DOL-1, DMI, and DFL carriers. The substitution at position 170 causes partial D although located deep in the vestibule.
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von Zabern, Inge
(author)
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Doescher, Andrea
(author)
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Wagner, Franz F
(author)
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Strathmann, Klaus P
(author)
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Geisen, Christof
(author)
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Plafi, MiodragÖstergötlands Läns Landsting,Linköpings universitet,Transfusionsmedicin,Hälsouniversitetet,Klinisk immunologi och transfusionsmedicin,Landstinget i Östergötland
(author)
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Pisacka, Martin
(author)
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Poole, Joyce
(author)
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Polin, Helene
(author)
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Gabriel, Christian
(author)
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Avent, Neil D
(author)
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University Hospital, UlmTransfusionsmedicin
(creator_code:org_t)
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In:Transfusion: Wiley49:6, s. 1059-10690041-11321537-2995
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Flegel, Willy A
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von Zabern, Inge
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Doescher, Andrea
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Wagner, Franz F
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Strathmann, Klau ...
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Geisen, Christof
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Plafi, Miodrag
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Pisacka, Martin
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Poole, Joyce
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Polin, Helene
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Gabriel, Christi ...
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Avent, Neil D
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Transfusion
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Linköping University