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Functionalized ZnO ...
Functionalized ZnO nanorod-based selective magnesium ion sensor for intracellular measurements
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- Asif, Muhammad (author)
- Linköpings universitet,Institutionen för teknik och naturvetenskap,Tekniska högskolan
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- Usman Ali, Syed (author)
- Linköpings universitet,Institutionen för teknik och naturvetenskap,Tekniska högskolan
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- Nour, Omer (author)
- Linköpings universitet,Institutionen för teknik och naturvetenskap,Tekniska högskolan
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- Willander, Magnus (author)
- Linköpings universitet,Institutionen för teknik och naturvetenskap,Tekniska högskolan
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- Englund, Ulrika (author)
- Linköpings universitet,Cellbiologi,Hälsouniversitetet
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- Elinder, Fredrik (author)
- Linköpings universitet,Cellbiologi,Hälsouniversitetet
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(creator_code:org_t)
- Elsevier Science B.V., Amsterdam. 2010
- 2010
- English.
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In: Biosensors & bioelectronics. - : Elsevier Science B.V., Amsterdam.. - 0956-5663 .- 1873-4235. ; 26:3, s. 1118-1123
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Abstract
Subject headings
Close
- ZnO nanorods were grown on a silver-coated tip of a borosilicate glass capillary (0.7 mu m in tip diameter) and used as selective potentiometric sensor of intracellular free Mg2+. To functionalize the ZnO nanorods for selectivity of Mg2+, a polymeric membrane with Mg2+-selective ionophores were coated on the surface of the ZnO nanorods. These functionalized ZnO nanorods exhibited a Mg2+-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode within the concentration range from 500 nM to 100 mM. Two types of cells, human adipocytes and frog oocytes, were used for the intracellular Mg2+ measurements. The intracellular concentration of free Mg2+ in human adipocytes and frog oocytes were 0.4-0.5 and 0.8-0.9 mM, respectively. Such type of nanoelectrode device paves the way to enable analytical measurements in single living cells and to sense other bio-chemical species at the intracellular level.
Keyword
- ZnO nanorods
- Potentiometric
- Mg2+-selective membrane
- Human adipocyte
- Frog oocyte
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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