Assessment of glucose oxidase behaviour in alcoholic solutions using disposable electrodes

Kroger, S (author): Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;

Setford, SJ (author): Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;

(creator_code:org_t)

Elsevier Science B.V., Amsterdam. 1998
1998
English.
In: Analytica Chimica Acta. - : Elsevier Science B.V., Amsterdam.. - 0003-2670 .- 1873-4324. ; 368:3, s. 219-231

Abstract Subject headings

Solvent resistant screen-printed three-electrode devices were employed to assess the behaviour of free and immobilised glucose oxidase in water-miscible organic solvent/aqueous buffer mixtures. Three alcoholic solvents were examined, methanol, ethanol and isopropanol. A rhodinised-carbon electrocatalyst was employed to facilitate hydrogen peroxide oxidation at a decreased operating potential. The devices had a linear response range of 1-10 mU GOX and allowed detection of down to 2 x 10(-14) mol GOX. The measurement of 0-1 (v/v) glucose in 30% (v/v) ethanol was achieved in 2 min using these highly storage-stable enzyme electrodes. Such devices are likely to have widespread applications in the diagnostic and on-line monitoring fields where rapid analysis in alcoholic matrices is desirable. (C) 1998 Elsevier Science B.V. All rights reserved.

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