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Ruthenized screen-printed choline oxidase-based biosensors for measurement of anticholinesterase activity
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- Cagnini, A (author)
- UNIV FLORENCE,DIPARTIMENTO SANITA PUBBL EPIDEMIOL and CHIM ANALIT,I-50121 FLORENCE,ITALY; CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND;
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- Palchetti, I (author)
- UNIV FLORENCE,DIPARTIMENTO SANITA PUBBL EPIDEMIOL and CHIM ANALIT,I-50121 FLORENCE,ITALY; CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND;
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- Mascini, M (author)
- UNIV FLORENCE,DIPARTIMENTO SANITA PUBBL EPIDEMIOL and CHIM ANALIT,I-50121 FLORENCE,ITALY; CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND;
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- (creator_code:org_t)
- Springer Science Business Media, 1995
- 1995
- English.
- In: Microchimica Acta. - : Springer Science Business Media. - 0026-3672 .- 1436-5073. ; 121:04-jan, s. 155-166
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Plastic disposable choline biosensors based on ruthenized-carbon screen-printed electrodes were prepared and their use for monitoring organophosphorus pesticides and carbamates is described. The presence of 0.5% ruthenium on activated carbon mixed to form a simple graphite-based ink for the working electrode surface increased the sensitivity towards hydrogen peroxide. The choline biosensor is based on such an electrode coupled with choline oxidase immobilized by adsorption and was used to detect the inhibition effect of carbamates and organophosphorus pesticides on acetylcholinesterase. With the optimized procedure described (pH, buffer composition, incubation time, substrate concentration), concentrations of pesticides (Carbofuran) as low as 1 nM could be detected.
Keyword
- choline sensor; amperometry; enzyme inhibition; thick film
- TECHNOLOGY
- TEKNIKVETENSKAP
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- ref (subject category)
- art (subject category)
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- Cagnini, A
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- Linköping University
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