ONLINE MONITORING OF GLUCOSE, GLUTAMATE AND GLUTAMINE DURING MAMMALIAN-CELL CULTIVATIONS

WHITE, SFGESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY; (author)

Amperometric biosensors (based on rhodinised carbon electrodes) for glucose, glutamine and glutamate were constructed. The sensors were incorporated into a three cell parallel FIA system and used to monitor the three analytes on-line during two mammalian cell perfusion cultures. All measurements were made simultaneously from undiluted media sample. Use of the FIA system enabled easy and rapid exchange of the sensors, during cultivation. The inclusion of a calibration step, regularly for all sensors, helped to maintain the accuracy of all measurements. Comparison with off-line measurements indicated that all three biosensors operated successfully, providing accurate information.

TURNER, APFCranfield University, UK(Swepub:liu)anttu13 (author)
BILTEWSKI, UGESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY; (author)
BRADLEY, JGESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY; (author)
SCHMID, RDGESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY; (author)
GESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY;Cranfield University, UK (creator_code:org_t)

In:Biosensors & bioelectronics: Elsevier Science B.V., Amsterdam.10:07-jun, s. 543-5510956-56631873-4235

By the author/editor: WHITE, SF; TURNER, APF; BILTEWSKI, U; BRADLEY, J; SCHMID, RD

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