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Freezing stallion s...
Freezing stallion semen with the new Caceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability
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- Morillo Rodriguez, A (author)
- University of Extremadura, Cáceres, Spain
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- Ortega Ferrusola, C (author)
- University of Extremadura, Cáceres, Spain
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- Macias Garcia, B (author)
- University of Extremadura, Cáceres, Spain
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- Morrell, Jane (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,Swedish University of Agriculture Science
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- Rodriguez-Martinez, Heriberto (author)
- Linköpings universitet,Hälsouniversitetet,Medicinsk genetik
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- Tapia, J A (author)
- University of Extremadura, Cáceres, Spain
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- Pena, F J (author)
- University of Extremadura, Cáceres, Spain
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(creator_code:org_t)
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- Elsevier Masson, 2011
- 2011
- English.
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In: Animal Reproduction Science. - : Elsevier Masson. - 0378-4320 .- 1873-2232. ; 127:1-2, s. 78-83
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https://liu.diva-por... (primary) (Raw object)
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https://doi.org/10.1...
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Abstract
Subject headings
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- Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytometry). Samples frozen in Caceres extender consistently showed the best results in post-thaw motility (increases ranging from 11 to 17%, p andlt; 0.05) and velocity (p andlt; 0.05), membrane integrity (increases ranging from 11 to 14%, p andlt; 0.05) and mitochondrial membrane potential (p andlt; 0.05). It is concluded that this new extender should be included in a freezeability test to determine the best extender for each individual.
Subject headings
- LANTBRUKSVETENSKAPER -- Veterinärmedicin (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science (hsv//eng)
- LANTBRUKSVETENSKAPER -- Husdjursvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Animal and Dairy Sience (hsv//eng)
Keyword
- Stallion
- Frozen sperm
- Flow cytometry
- CASA
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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