Angiogenesis from a new perspective

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: onr:"swepub:oai:DiVA.org:liu-73137" > Angiogenesis from a...

1 of 1
Previous record
Next record
To hitlist

Angiogenesis from a new perspective

Bourghardt Peebo, Beatrice, 1968- (author): Östergötlands Läns Landsting,Linköpings universitet,Oftalmiatrik,Hälsouniversitetet,Ögonkliniken US/LiM

Lagali, Neil, Dr. (thesis advisor): Östergötlands Läns Landsting,Linköpings universitet,Oftalmiatrik,Hälsouniversitetet,Ögonkliniken US/LiM

Fagerholm, Per, Professor (thesis advisor): Östergötlands Läns Landsting,Linköpings universitet,Oftalmiatrik,Hälsouniversitetet,Ögonkliniken US/LiM

Behndig, Anders (opponent): Umeå universitet, Klinisk vetenskap/Oftalmiatrik, Umeå, Sverige

show less...

(creator_code:org_t)

ISBN 9789175199993
Linköping : Linköping University Electronic Press, 2012
English 91 s.
Series: Linköping University Medical Dissertations, 0345-0082 ; 1284

Related links:: https://liu.diva-por... (primary) (Raw object); show more...; https://liu.diva-por...; https://urn.kb.se/re...; show less...

Doctoral thesis (other academic/artistic)

Abstract Subject headings

Angiogenesis is the emergence of new blood and lymph vessels from existing ones. In the pathologic form it contributes to the onset and progression of numerous different human disorders such as cancer, inflammation, atherosclerosis and blinding eye diseases. There exist a number of models to study angiogenesis, both in vitro and in vivo, but there is no single perfect model so far. Consequently there is a need to develop new angiogenesis assays for evaluating blood and lymph vessel behaviour in different physiologic settings.The aim of this thesis was to gain insight into in vivo angiogenesis introducing a new technique in an inflammatory corneal model. The method involved in vivo examination of the cornea and subsequent comparison of in vivo findings with ex vivo immunohistochemical analysis of the same tissue samples. An existing suture model for inflammatory angiogenesis in the cornea was modified for in vivo observations with a clinically-approved corneal confocal microscope.In this thesis, corneal lymph vessels were characterized for the first time in vivo and findings from the experimental bench could be applied in a clinical setting, where presumed lymphatics were observed in a corneal transplant patient with rejection. Furthermore, the technique was extended to investigate time-lapse processes in sprouting and regressing capillaries, and led to a number of new observations. CD11b+ myeloid cells constitute the first bulk of infiltrating inflammatory cells and contribute to inflammatory sprouting and regression in numerous ways including pre-patterning of the corneal stroma and guiding of capillary sprouts. Newly formed hemangiogenic sprouts are perfused with a slow-moving fluid and have a lumen. In blood vessel regression, capillary remodeling occurred by abandonment of sprout tips in close association with macrophages and vascular loops formed by presumed intussusceptive angiogenesis. In addition, a network of pericyte- and endothelium-free basement membrane tubes was formed after desertion or degradation of vascular endothelium in former corneal capillaries.In conclusion, we introduce a new in vivo technique for investigating angiogenesis in a corneal model were in vivo findings can be interpreted with ex vivo definitions of specific cell types by immunohistochemistry. Findings from pre-clinical experiments have been possible to apply in a clinical setting when examining patients with corneal pathology.

Publication and Content Type

vet (subject category)
dok (subject category)

Find in a library

Angiogenesis from a new perspective (Search the publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Bourghardt Peebo ...; Lagali, Neil, Dr ...; Fagerholm, Per, ...; Behndig, Anders

Parts in the series: Linköping Univer ...

By the university: Linköping University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se