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- Zhou, BinSichuan University (author)
PNAS-4 expression and its relationship to p53 in colorectal cancer
- Article/chapterEnglish2012
Publisher, publication year, extent ...
- 2011-05-10
- Springer Verlag (Germany),2012
- printrdacarrier
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- LIBRIS-ID:oai:DiVA.org:liu-73308
- https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-73308URI
- https://doi.org/10.1007/s11033-011-0732-3DOI
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- Language:English
- Summary in:English
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- PNAS-4 is a novel pro-apoptotic protein activated during the early response to DNA damage; however, the molecular mechanisms and pathways regulating PNAS-4 expression in tumors are not well understood. We hypothesized that PNAS-4 is a p53 down-stream target gene and designed this study. We searched online for putative p53-binding sites in the entire PNAS-4 gene and did not find any corresponding information. In HCT116 colon cancer cells, after being transfected with small interfering RNA to silence p53, the expressions of PNAS-4 and other known p53 target gene (Apaf1, Bax, Fas and Dr5) were determined by real-time PCR. We found that PNAS-4 was up-regulated while Apaf1, Bax, Fas and Dr5 were down-regulated. We then examined the expression of PNAS-4 and p53 mutation in colorectal cancer patients. PNAS-4 expressed both in colorectal cancers and normal tissues, but compared with paired control, PNAS-4 was up-regulated in cancers (P = 0.018). PNAS-4 overexpression ratios were correlated to the p53 mutant status (P = 0.001). The mean PNAS-4 expression levels of p53 mutant homozygote group and heterozygote group were higher than that of p53 wild type group (P = 0.013). The expression ratios of PNAS-4 (every sample in relative to its paired normal mucosa) were different between negative lymph node metastasis (66% up-regulated, 34% down-regulated) and positive metastasis (42% up-regulated, 58% down-regulated). Taken together, these findings suggested that PNAS-4 was not a p53 target, but overexpression of PNAS-4 was correlated to p53 inactivity in colorectal cancer.
Subject headings and genre
- Colorectal carcinoma; PNAS-4; Small interfering RNA; p53; Real-time PCR
- MEDICINE
- MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
- Yan, HuiSichuan University (author)
- Li, YuanSichuan University (author)
- Wang, RongSichuan University (author)
- Chen, KelingSichuan University (author)
- Zhou, ZongguangSichuan University (author)
- Sun, Xiao-FengÖstergötlands Läns Landsting,Linköpings universitet,Onkologi,Hälsouniversitetet,Onkologiska kliniken US(Swepub:liu)xiasu45 (author)
- Sichuan UniversityOnkologi (creator_code:org_t)
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- In:Molecular Biology Reports: Springer Verlag (Germany)39:1, s. 243-2490301-48511573-4978
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