Membrane Lipids of the Stallion Spermatozoon in Relation to Sperm Quality and Susceptibility to Lipid Peroxidation

Macias Garcia, B. (author): Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura, Cáceres, Spain

Gonzalez Fernandez, L. (author): Department of Physiology, University of Extremadura, Avd de la Universidad s/n, Cáceres, Spain

Ortega Ferrusola, C. (author): Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura, Cáceres, Spain

Salazar-Sandoval, C. (author): Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura, Cáceres, Spain

Morillo Rodriguez, A. (author): Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura, Cáceres, Spain

Rodriguez-Martinez, Heriberto (author): Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Linköpings universitet,Utvecklingsbiologi,Hälsouniversitetet,Division of Reproduction, Faculty of Veterinary Medicine and Animal Sciences, SLU, Uppsala, Sweden,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences

Tapia, J.A. (author): Department of Physiology, University of Extremadura, Avd de la Universidad s/n, Cáceres, Spain

Morcuende, D. (author): Department of Animal Production and Food Science Faculty of Veterinary Medicine, University of Extremadura, Avd de la Universidad s/n, Cáceres, Spain

Pena, F.J. (author): Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura, Cáceres, Spain

(creator_code:org_t)

2011-01-18
2011
English.
In: Reproduction in domestic animals. - : Blackwell Publishing. - 0936-6768 .- 1439-0531. ; 46:1, s. 141-148

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; https://res.slu.se/i...; show less...

Abstract Subject headings

Contents: Lipids were extracted from ejaculated spermatozoa from seven individual stallions to distinguish neutral lipids (NL) and polar lipids (PL) and determine their variation among stallions and their relationship with sperm quality and sperm susceptibility to lipid peroxidation. The isolated fatty acids were correlated with sperm quality (membrane integrity, mitochondrial membrane potential (ΔΨm) and expression of active caspases) and the sensitivity of the sperm plasma membrane to LPO. The miristic (C14: 0), palmitic (C16: 0), stearic (C18: 0) and oleic (C18: 1n9) acids were predominant among the NLs. Within the phospholipid fraction, the docosapentanoic acid (C22: 5n6) was dominant, albeit varying among stallions. Surprisingly, the percentage of polyunsaturated fatty acids was positively correlated with sperm quality and a low propensity for LPO, probably because these particular fatty acids provide a higher fluidity of the plasma membrane. The stallion showing the poorest sperm membrane integrity plus a high level of LPO in his ejaculate had a lower percentage (p less than 0.05) of this fatty acid in his sperm plasma membranes. © 2010 Blackwell Verlag GmbH.

By the author/editor: Macias Garcia, B ...; Gonzalez Fernand ...; Ortega Ferrusola ...; Salazar-Sandoval ...; Morillo Rodrigue ...; Rodriguez-Martin ...; show more...; Tapia, J.A.; Morcuende, D.; Pena, F.J.; show less...

About the subject

By the university: Linköping University; Swedish University of Agricultural Sciences

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