onr:"swepub:oai:DiVA.org:liu-75743"
Search: onr:"swepub:oai:DiVA.org:liu-75743" > An Auto-Catalytic S...
- 1 of 1
- Previous record
- Next record
- To hitlist
An Auto-Catalytic Surface for Conformational Replication of Amyloid Fibrils-Genesis of an Amyloid World?
-
- Hammarström, Per (author)
- Linköpings universitet,Biokemi,Tekniska högskolan
-
- Ali, Malik M (author)
- Linköpings universitet,Institutionen för fysik, kemi och biologi,Tekniska högskolan
-
- Mishra, Rajesh (author)
- Linköpings universitet,Biokemi,Tekniska högskolan
- show more...
- show less...
- (creator_code:org_t)
- 2010-12-03
- 2011
- English.
- In: Origins of life and evolution of the biosphere. - : Springer Verlag (Germany). - 0169-6149 .- 1573-0875. ; 41:4, s. 373-383
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Amyloid fibrils are composed of self assembled stacked peptide or protein molecules folded and trapped in a stable cross-beta-sheet conformation. The amyloid fibrillation mechanism represents an intriguing self-catalyzed process rendering replication of a molecular conformational memory of interest for prebiotic chemistry. Herein we describe how a solid surface can be rendered auto-catalytic for fibrillation of a protein solution. We have discovered that a hydrophobic silicon or glass surface can be made to continuously fibrillate solutions of insulin monomers under stressed conditions (pH 1.6, 65 degrees C). It was found that the surface acts as a platform for the formation of nascent seeds that induce fibril replication on and at the surface. This autocatalytic effect stems from a layer a few insulin molecules thick representing an oligomeric layer of misfolded, conformationally trapped, insulin molecules that rapidly through epitaxial growth catalyze the rate determining step (nucleation) during fibril replication. This autocatalytic layer is generated by the protein-solid surface interaction and conformational changes of the adsorbed protein during exposure at the air-water interface. The resulting autocatalytic surface thus both initiates local conformational molecular self-replication and acts as a reservoir for fibril seeds budding off into solution spreading fibril replication entities to the surrounding medium. The possibility of catalysis of the conformational replication process by minute amounts of nucleation sites located on a recruiting surface can evade the issue of dramatic concentration dependence of amyloidogenesis.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Keyword
- Amyloid
- Molecular replication
- Self-assembly
- Origin of life
- TECHNOLOGY
- TEKNIKVETENSKAP
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Origins of life and evolution of the biosphere (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Hammarström, Per
- Ali, Malik M
- Mishra, Rajesh
- Salagic, Belma
- Svensson, Samuel
- Tengvall, Pentti
- show more...
- Lundström, Ingem ...
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Clinical Medicin ...
- and Dentistry
- Articles in the publication
- Origins of life ...
- By the university
- Linköping University
- University of Gothenburg
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
