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An Electrochemical ...
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Al-Khafaji, Q A MUniversity of Florence
(author)
An Electrochemical Immunoassay for HER2 Detection
- Article/chapterEnglish2012
Publisher, publication year, extent ...
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2012-01-12
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Wiley-VCH Verlag Berlin,2012
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:liu-76937
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-76937URI
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https://doi.org/10.1002/elan.201100501DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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In this paper, a simple and sensitive approach for human epidermal growth factor receptor 2 (HER2) detection is presented, using antibody-functionalised magnetic beads coupled to screen-printed cells. The immunoassay is based on a sandwich format in which a primary monoclonal antibody anti-HER2 is coupled to protein A modified magnetic beads. The modified beads are then used to capture the protein from the sample solution and a sandwich assay is performed by adding a secondary monoclonal antibody anti-HER2 labelled with biotin. The enzyme alkaline phosphatase (AP) conjugated with streptavidin and its substrate (1-naphthyl-phosphate) are then used for the electrochemical detection by differential pulse voltammetry (DPV). The experimental conditions for the immunoassay were optimised. The performance of the assay in terms of sensitivity, reproducibility and selectivity has been studied in buffer and serum samples from hospital patients.
Subject headings and genre
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HER2
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Cancer marker
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Magnetic beads
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Immunoassay
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TECHNOLOGY
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TEKNIKVETENSKAP
Added entries (persons, corporate bodies, meetings, titles ...)
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Harris, MUniversity of Florence
(author)
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Tombelli, SUniversity of Florence
(author)
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Laschi, SUniversity of Florence
(author)
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Turner, AnthonyLinköpings universitet,Biosensorer och bioelektronik,Tekniska högskolan(Swepub:liu)anttu13
(author)
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Mascini, MUniversity of Florence
(author)
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Marrazza, GUniversity of Florence
(author)
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University of FlorenceBiosensorer och bioelektronik
(creator_code:org_t)
Related titles
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In:Electroanalysis: Wiley-VCH Verlag Berlin24:4, s. 735-7421040-03971521-4109
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