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Coupling of α1-adre...
Coupling of α1-adrenoceptors to ERK1/2 in the human prostate
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- Bauer, Ricarda M. (author)
- University of Munich, Germany
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- Strittmatter, Frank (author)
- University of Munich, Germany
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- Gratzke, Christian (author)
- University of Munich, Germany
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- Göttinger, Johanna (author)
- University of Munich, Germany
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- Schlenker, Boris (author)
- University of Munich, Germany
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- Reich, Oliver (author)
- University of Munich, Germany
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- Stief, Christian G. (author)
- University of Munich, Germany
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- Hedlund, Petter (author)
- Linköpings universitet,Klinisk farmakologi,Hälsouniversitetet
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- Andersson, Karl-Erik (author)
- Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, N.C., USA
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- Hennenberg, Martin (author)
- University of Munich, Germany
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(creator_code:org_t)
- 2011-02-03
- 2011
- English.
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In: Urologia internationalis. - : S. Karger AG. - 0042-1138 .- 1423-0399. ; 86:4, s. 427-433
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Abstract
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- INTRODUCTION: α1-Adrenoceptors are considered critical for the regulation of prostatic smooth muscle tone. However, previous studies suggested further α1-adrenoceptor functions besides contraction. Here, we investigated whether α1-adrenoceptors in the human prostate may activate extracellular signal-regulated kinases (ERK1/2). METHODS: Prostate tissues from patients undergoing radical prostatectomy were stimulated in vitro. Activation of ERK1/2 was assessed by Western blot analysis. Expression of ERK1/2 was studied by immunohistochemistry. The effect of ERK1/2 inhibition by U0126 on phenylephrine-induced contraction was studied in organ-bath experiments. RESULTS: Stimulation of human prostate tissue with noradrenaline (30 μM) or phenylephrine (10 μM) resulted in ERK activation. This was reflected by increased levels of phosphorylated ERK1/2. Expression of ERK1/2 in the prostate was observed in smooth muscle cells. Incubation of prostate tissue with U0126 (30 μM) resulted in ERK1/2 inhibition. Dose-dependent phenylephrine-induced contraction of prostate tissue was not modulated by U0126. CONCLUSIONS: α1-Adrenoceptors in the human prostate are coupled to ERK1/2. This may partially explain previous observations suggesting a role of α1-adrenoceptors in the regulation of prostate growth.
Keyword
- Prostate hyperplasia
- 1-Adrenoceptor
- Extracellular signal-regulated kinase
- Smooth muscle
- Lower urinary tract symptoms
- α1-Adrenoceptor blocker
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- ref (subject category)
- art (subject category)
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Bauer, Ricarda M ...
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Strittmatter, Fr ...
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Gratzke, Christi ...
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Göttinger, Johan ...
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Schlenker, Boris
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Reich, Oliver
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show more...
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Stief, Christian ...
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Hedlund, Petter
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Andersson, Karl- ...
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Hennenberg, Mart ...
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Urologia interna ...
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Linköping University