Combined Multivariate and Pathway Analyses Show That Allergen-Induced Gene Expression Changes in CD4(+) T Cells Are Reversed by Glucocorticoids

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: onr:"swepub:oai:DiVA.org:liu-79798" > Combined Multivaria...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Zhao, YelinLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet (author)

Combined Multivariate and Pathway Analyses Show That Allergen-Induced Gene Expression Changes in CD4(+) T Cells Are Reversed by Glucocorticoids

Article/chapterEnglish2012

Publisher, publication year, extent ...

2012-06-12
Public Library of Science,2012
electronicrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:liu-79798
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-79798URI
https://doi.org/10.1371/journal.pone.0039016DOI
https://gup.ub.gu.se/publication/160363URI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Background: Glucocorticoids (GCs) play a key role in the treatment of allergy. However, the genome-wide effects of GCs on gene expression in allergen-challenged CD4(+) T cells have not been described. The aim of this study was to perform a genome-wide analysis to investigate whether allergen-induced gene expression changes in CD4(+) T cells could be reversed by GCs. Methodology/Principal Findings: Gene expression microarray analysis was performed to profile gene expression in diluent( D), allergen- (A), and allergen + hydrocortisone- (T) challenged CD4(+) T cells from patients with seasonal allergic rhinitis. Principal component analysis (PCA) showed good separation of the three groups. To identify the correlation between changes in gene expression in allergen-challenged CD4(+) T cells before and after GC treatment, we performed orthogonal partial least squares discriminant analysis (OPLS-DA) followed by Pearson correlation analysis. This revealed that allergen-induced genes were widely reversed by GC treatment (r = -0.77, Pless than0.0001). We extracted 547 genes reversed by GC treatment from OPLS-DA models based on their high contribution to the discrimination and found that those genes belonged to several different inflammatory pathways including TNFR2 Signalling, Interferon Signalling, Glucocorticoid Receptor Signalling and T Helper Cell Differentiation. The results were supported by gene expression microarray analyses of two independent materials. Conclusions/Significance: Allergen-induced gene expression changes in CD4(+) T cells were reversed by treatment with glucocorticoids. The top allergen-induced genes that reversed by GC treatment belonged to several inflammatory pathways and genes of known or potential relevance for allergy.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Pediatrik hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Pediatrics hsv//eng
MEDICINE
MEDICIN

Added entries (persons, corporate bodies, meetings, titles ...)

Wang, HuiGothenburg University,Göteborgs universitet,Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics(Swepub:gu)xwanhu (author)
Gustafsson, MikaLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:liu)mikgu75 (author)
Muraro, AntonellaUniversity of Padua, Italy (author)
Bruhn, SörenLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:liu)sorbr27 (author)
Benson, MikaelLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:gu)xbenmi (author)
Linköpings universitetInstitutionen för klinisk och experimentell medicin (creator_code:org_t)

Related titles

In:PLOS ONE: Public Library of Science7:61932-6203

Internet link

Find in a library

PLOS ONE (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Zhao, Yelin; Wang, Hui; Gustafsson, Mika; Muraro, Antonell ...; Bruhn, Sören; Benson, Mikael

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Pediatrics

Articles in the publication: PLOS ONE

By the university: Linköping University; University of Gothenburg

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se