Search: onr:"swepub:oai:DiVA.org:liu-79798" >
Combined Multivaria...
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Zhao, YelinLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet
(author)
Combined Multivariate and Pathway Analyses Show That Allergen-Induced Gene Expression Changes in CD4(+) T Cells Are Reversed by Glucocorticoids
- Article/chapterEnglish2012
Publisher, publication year, extent ...
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2012-06-12
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Public Library of Science,2012
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:liu-79798
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-79798URI
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https://doi.org/10.1371/journal.pone.0039016DOI
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https://gup.ub.gu.se/publication/160363URI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Background: Glucocorticoids (GCs) play a key role in the treatment of allergy. However, the genome-wide effects of GCs on gene expression in allergen-challenged CD4(+) T cells have not been described. The aim of this study was to perform a genome-wide analysis to investigate whether allergen-induced gene expression changes in CD4(+) T cells could be reversed by GCs. Methodology/Principal Findings: Gene expression microarray analysis was performed to profile gene expression in diluent( D), allergen- (A), and allergen + hydrocortisone- (T) challenged CD4(+) T cells from patients with seasonal allergic rhinitis. Principal component analysis (PCA) showed good separation of the three groups. To identify the correlation between changes in gene expression in allergen-challenged CD4(+) T cells before and after GC treatment, we performed orthogonal partial least squares discriminant analysis (OPLS-DA) followed by Pearson correlation analysis. This revealed that allergen-induced genes were widely reversed by GC treatment (r = -0.77, Pless than0.0001). We extracted 547 genes reversed by GC treatment from OPLS-DA models based on their high contribution to the discrimination and found that those genes belonged to several different inflammatory pathways including TNFR2 Signalling, Interferon Signalling, Glucocorticoid Receptor Signalling and T Helper Cell Differentiation. The results were supported by gene expression microarray analyses of two independent materials. Conclusions/Significance: Allergen-induced gene expression changes in CD4(+) T cells were reversed by treatment with glucocorticoids. The top allergen-induced genes that reversed by GC treatment belonged to several inflammatory pathways and genes of known or potential relevance for allergy.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Wang, HuiGothenburg University,Göteborgs universitet,Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics(Swepub:gu)xwanhu
(author)
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Gustafsson, MikaLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:liu)mikgu75
(author)
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Muraro, AntonellaUniversity of Padua, Italy
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Bruhn, SörenLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:liu)sorbr27
(author)
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Benson, MikaelLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:gu)xbenmi
(author)
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Linköpings universitetInstitutionen för klinisk och experimentell medicin
(creator_code:org_t)
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In:PLOS ONE: Public Library of Science7:61932-6203
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