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A pathway-based app...
A pathway-based approach to find novel markers of local glucocorticoid treatment in intermittent allergic rhinitis
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- Wang, Hui (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics,University of Gothenburg, Sweden
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- Chavali, Sreenivas (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics,University of Gothenburg, Sweden
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- Mobini, Reza, 1965 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics,University of Gothenburg, Sweden
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- Muraro, A. (author)
- University of Padua, Italy
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- Barbon, F. (author)
- University of Padua, Italy
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- Boldrin, D. (author)
- University of Padua, Italy
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- Åberg, Nils, 1943 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics,The Queen Silvia Children’s Hospital, Gothenburg, Sweden
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- Benson, Mikael (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics,The Queen Silvia Children’s Hospital, Gothenburg, Sweden
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(creator_code:org_t)
- 2010-12-03
- 2011
- English.
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In: Allergy. European Journal of Allergy and Clinical Immunology. - : Wiley-Blackwell. - 0105-4538 .- 1398-9995. ; 66:1, s. 132-140
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- BACKGROUND: Glucocorticoids (GCs) may affect the expression of hundreds of genes in different cells and tissues from patients with intermittent allergic rhinitis (IAR). It is a formidable challenge to understand these complex changes by studying individual genes. In this study, we aimed to identify (i) pathways affected by local GC treatment and (ii) examine if those pathways could be used to find novel markers of local GC treatment in nasal fluids from patients with IAR. METHODS: Gene expression microarray- and iTRAQ-based proteomic analyses of nasal fluids, nasal fluid cells and nasal mucosa from patients with IAR were performed to find pathways enriched for differentially expressed genes and proteins. Proteins representing those pathways were analyzed with ELISA in an independent material of nasal fluids from 23 patients with IAR before and after treatment with a local GC. RESULTS: Transcriptomal and proteomic high-throughput analyses of nasal fluids, nasal fluid cells and nasal mucosal showed that local GC treatment affected a wide variety of pathways in IAR such as the glucocorticoid receptor pathway and the acute phase response pathway. Extracellular proteins encoded by genes in those pathways were analyzed in an independent material of nasal fluids from patients. Proteins that changed significantly in expression included known biomarkers such as eosinophil cationic protein but also proteins that had not been previously described in IAR, namely CCL2, M-CSF, CXCL6 and apoH. CONCLUSION: Pathway-based analyses of genomic and proteomic high-throughput data can be used as a complementary approach to identify novel potential markers of GC treatment in IAR.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Adult
- Biological Markers/*metabolism
- Female
- Gene Expression Profiling
- Genomics
- Glucocorticoids/*therapeutic use
- High-Throughput Screening Assays
- Humans
- Male
- Nasal Lavage Fluid/immunology
- Nasal Mucosa/immunology/metabolism
- *Oligonucleotide Array Sequence Analysis
- *Proteomics
- Rhinitis
- Allergic
- Seasonal/*drug therapy/metabolism
- Young Adult
- allergic rhinitis; gene expression microarray
- analysis; glucocorticoids; proteomics
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Wang, Hui
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Chavali, Sreeniv ...
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Mobini, Reza, 19 ...
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Muraro, A.
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Barbon, F.
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Boldrin, D.
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show more...
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Åberg, Nils, 194 ...
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Benson, Mikael
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
- Articles in the publication
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Allergy. Europea ...
- By the university
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Linköping University
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University of Gothenburg