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Characterization of...
Characterization of α2-Adrenoceptor Subtypes in Pregnant Human Myometrium
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- Adolfsson, Per I. (author)
- Linköpings universitet,Farmakologi,Hälsouniversitetet
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- Dahle, Lars Olav (author)
- Linköpings universitet,Obstetrik och gynekologi,Hälsouniversitetet
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- Berg, Göran (author)
- Linköpings universitet,Obstetrik och gynekologi,Hälsouniversitetet
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- Svensson, Samuel P. S. (author)
- Linköpings universitet,Farmakologi,Hälsouniversitetet
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(creator_code:org_t)
- 1998-04-03
- 1998
- English.
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In: Gynecologic and Obstetric Investigation. - : S. Karger AG. - 0378-7346 .- 1423-002X. ; 45:3, s. 145-150
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The aim of the present investigation was to determine which subtypes of the α2-adrenoceptors are being expressed in the human pregnant myometrium at term pregnancy. In radioligand binding studies, the specific binding of [3H]rauwolscine to human myometrial membranes was specific and of high affinity with Kd of 2.8 ± 0.6 nM and Bmax of 95 ± 5 fmol/mg protein. Results from competition for the binding of [3H]rauwolscine using subtype-selective ligands, oxymetazoline (α2A-subptype), chlorpromazine (α2B-subtype) and prazosin (α2B-α2C-subtype), suggested that the α2A- and α2B-subtypes are being co-expressed. In order to examine if also the α2C-subtype is being expressed we used an optimal concentration of oxymetazoline or chlorpromazine which would block the high-affinity site, equivalent to the α2A- and α2B-subtype respectively. Competition curves of both oxymetazoline and chlorpromazine still showed a significantly better fit using a two-site model, suggesting that the α2C-subtype also is being expressed. The expression of α2C-subtype mRNA was confirmed using reverse transcription-polymerase chain reaction on mRNA isolated from myometrial biopsies.In conclusion, our results suggest that all three subtypes of α2-adrenoceptors are being coexpressed in the human myometrium at term pregnancy and that α2-expression is dominated by the α2A-subtype.
Keyword
- α2-Adrenergic receptors
- Subtype
- Myometrium
- Human
- Pregnancy
- [3H]rauwolscine
- Reverse transcription-polymerase chain reaction
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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