SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:DiVA.org:liu-85099"
 

Search: onr:"swepub:oai:DiVA.org:liu-85099" > Over-expression of ...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Eklund, Lena K.Linköpings universitet,Cellbiologi,Hälsouniversitetet (author)

Over-expression of coronin 2a and lack of alterations in transforming growth factor ß receptor I in squamous cell carsinomas of the skin

  • BookEnglish

Publisher, publication year, extent ...

  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:liu-85099
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-85099URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:vet swepub-contenttype
  • Subject category:ovr swepub-publicationtype

Notes

  • Allelic losses in several regions of chromosome 9q have been connected to the development of squamous cell carcinoma (SCC) of the skin. We have studied two candidate genes in the 9q22 region using mutational analysis of genomic DNA as well as immunohistochemistry for assessment of changes in protein expression. The coronin 2A (CORO2A) protein shows strong resemblance to actin-binding proteins, implying a role in cytokinesis or cell motility. It has also been found to be part of the nuclear receptor co-repressor complex involved in transcriptional regulation. We elucidated the exon-intron structure by sequence alignment of the mRNA to a "high-throughput genomic sequence" entry in GenBank. By using single strand conformation analysis and DNA sequencing we found eight silent mutations in tumor DNA, one of which was found in a subset of a normal control population. Surprisingly, immunostaining revealed over-expression in 4/40 tumors. This cannot explain the high frequency of allelic loss in cutaneous secs, but is yet indicating a possible involvement of CORO2A in cutaneous SCC development. The gene for transforming growth factor ß receptor 1 (TßR-I) has previously been positioned to the 9q22 region. TßR-I is part of a protein complex necessary for binding of the TGFß ligand initiating a signaling cascade, which affects downstream targets important for cell cycle regulation. We could not identify any alterations at either protein or DNA level and therefore exclude TßR-I as candidate for cutaneous sec development.

Added entries (persons, corporate bodies, meetings, titles ...)

  • Undén, Anne BirgitteKarolinska Intitute, Department of Biosciences at Novum, Center for Nutrition and Toxicology, Huddinge, Sweden and Department of Dermatology, Karolinska Hospital, Stockholm, Sweden (author)
  • Lundin, Kristing B.Linköpings universitet,Cellbiologi,Hälsouniversitetet (author)
  • Zaphiropoulos, Peter G.Karolinska Institute, Department of Biosciences at Novum, Center for Nutrition and Toxicology, Huddinge, Sweden (author)
  • Toftgård, RuneKarolinska Institute, Department of Biosciences at Novum, Center for Nutrition and Toxicology, Huddinge, Sweden (author)
  • Söderkvist, PeterLinköpings universitet,Cellbiologi,Hälsouniversitetet(Swepub:liu)petso43 (author)
  • Linköpings universitetCellbiologi (creator_code:org_t)

Internet link

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view