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Opposing Effects of...
Opposing Effects of Circadian Clock Genes Bmal1 and Period2 in Regulation of VEGF-Dependent Angiogenesis in Developing Zebrafish
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- Dahl Jensen, Lasse (author)
- Karolinska Institutet,Linköpings universitet,Farmakologi,Hälsouniversitetet
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- Cao, Ziquan (author)
- Linköpings universitet,Avdelningen för kardiovaskulär medicin,Hälsouniversitetet
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- Nakamura, Masaki (author)
- Karolinska Institutet
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- Yang, Yunlong (author)
- Karolinska Institutet
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- Brautigam, Lars (author)
- Karolinska Institutet
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- Andersson, Patrik (author)
- Karolinska Institutet
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- Zhang, Yin (author)
- Karolinska Institute, Sweden
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- Wahlberg, Eric (author)
- Östergötlands Läns Landsting,Linköpings universitet,Kärlkirurgi,Hälsouniversitetet,Thorax-kärlkliniken i Östergötland
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- Länne, Toste (author)
- Östergötlands Läns Landsting,Linköpings universitet,Fysiologi,Hälsouniversitetet,Thorax-kärlkliniken i Östergötland
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- Hosaka, Kayoko (author)
- Karolinska Institutet
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- Cao, Yihai (author)
- Linköpings universitet,Avdelningen för kardiovaskulär medicin,Hälsouniversitetet,Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden
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(creator_code:org_t)
- Elsevier (Cell Press), 2012
- 2012
- English.
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In: Cell Reports. - : Elsevier (Cell Press). - 2211-1247. ; 2:2, s. 231-241
- Related links:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
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- Molecular mechanisms underlying circadian-regulated physiological processes remain largely unknown. Here, we show that disruption of the circadian clock by both constant exposure to light and genetic manipulation of key genes in zebrafish led to impaired developmental angiogenesis. A bmal1-specific morpholino inhibited developmental angiogenesis in zebrafish embryos without causing obvious nonvascular phenotypes. Conversely, a period2 morpholino accelerated angiogenic vessel growth, suggesting that Bmal1 and Period2 display opposing angiogenic effects. Using a promoter-reporter system consisting of various deleted vegf-promoter mutants, we show that Bmal1 directly binds to and activates the vegf promoter via E-boxes. Additionally, we provide evidence that knockdown of Bmal1 leads to impaired Notch-inhibition-induced vascular sprouting. These results shed mechanistic insight on the role of the circadian clock in regulation of developmental angiogenesis, and our findings may be reasonably extended to other types of physiological or pathological angiogenesis.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Dahl Jensen, Las ...
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Cao, Ziquan
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Nakamura, Masaki
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Yang, Yunlong
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Brautigam, Lars
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Andersson, Patri ...
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show more...
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Zhang, Yin
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Wahlberg, Eric
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Länne, Toste
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Hosaka, Kayoko
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Cao, Yihai
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show less...
- Articles in the publication
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Cell Reports
- By the university
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Linköping University
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Karolinska Institutet