Search: onr:"swepub:oai:DiVA.org:liu-86916" >
Prolonged Survival ...
-
Alberti, EstebanDepartment of Neurobiology, International Center of Neurological Restoration, CIREN, Havana, Cuba.
(author)
Prolonged Survival and expression of neural markers by bone marrow-derived stem cells transplanted into brain lesions
- Article/chapterEnglish2009
Publisher, publication year, extent ...
-
2009
-
electronicrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:liu-86916
-
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-86916URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
BACKGROUND: Bone marrow-derived stem cell transplantation is a potentially viable therapeutic option for the treatment of neurodegenerative disease. MATERIAL/METHODS: We have isolated bone marrow stem cells by standard method. We then evaluated the survival of rats' bone marrow mononuclear cells implanted in rats' brain. The cells were extracted from rats' femurs, and marked for monitoring purposes by adenoviral transduction with Green Fluorescent Protein (GFP). Labeled cells were implanted within the area of rats' striatum lesions that were induced a month earlier employing quinolinic acid-based method. The implants were phenotyped by monitoring CD34; CD38; CD45 and CD90 expression. Bone marrow stromal cells were extracted from rats' femurs and cultivated until monolayer bone marrow stromal cells were obtained. The ability of bone marrow stromal cells to express NGF and GDNF was evaluated by RT-PCR. RESULTS: Implanted cells survived for at least one month after transplantation and dispersed from the area of injection towards corpus callosum and brain cortex. Interestingly, passaged rat bone marrow stromal cells expressed NGF and GDNF mRNA. CONCLUSIONS: The bone marrow cells could be successfully transplanted to the brain either for the purpose of trans-differentiation, or for the expression of desired growth factors.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Los, Marek JanInterfaculty Institute for Biochemistry, University of Tübingen, Germany; BioApplications Enterprises, Winnipeg, MB, Canada(Swepub:liu)Marlo14
(author)
-
Garcia, RocioDepartment of Neurobiology, International Center of Neurological Restoration, CIREN, Havana, Cuba.
(author)
-
Fraga, JLDepartment of Neurobiology, International Center of Neurological Restoration, CIREN, Havana, Cuba.
(author)
-
Serrano, T.Department of Neurobiology, International Center of Neurological Restoration, CIREN, Havana, Cuba.
(author)
-
Hernandez, E.Department of Neurobiology, International Center of Neurological Restoration, CIREN, Havana, Cuba.
(author)
-
Klonisch, ThomasDepartment of Human Anatomy and Cell Sciences, and Manitoba Institute of Child Health, Winnipeg, Canada
(author)
-
Macías, R.Department of Neurobiology, International Center of Neurological Restoration, CIREN, Havana, Cuba.
(author)
-
Martinez, L.Department of Neurobiology, International Center of Neurological Restoration, CIREN, Havana, Cuba.
(author)
-
Castillo, L.Department of Neurobiology, International Center of Neurological Restoration, CIREN, Havana, Cuba.
(author)
-
de la Cuétara, K.Department of Neurobiology, International Center of Neurological Restoration, CIREN, Havana, Cuba
(author)
-
Department of Neurobiology, International Center of Neurological Restoration, CIREN, Havana, Cuba.Interfaculty Institute for Biochemistry, University of Tübingen, Germany; BioApplications Enterprises, Winnipeg, MB, Canada
(creator_code:org_t)
Related titles
-
In:Medical Science Monitor15:2, s. BR47-BR541234-10101643-3750
Internet link
Find in a library
To the university's database
- By the author/editor
-
Alberti, Esteban
-
Los, Marek Jan
-
Garcia, Rocio
-
Fraga, JL
-
Serrano, T.
-
Hernandez, E.
-
show more...
-
Klonisch, Thomas
-
Macías, R.
-
Martinez, L.
-
Castillo, L.
-
de la Cuétara, K ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Medical Biotechn ...
-
and Medical Biotechn ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biochemistry and ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Cell Biology
- Articles in the publication
-
Medical Science ...
- By the university
-
Linköping University