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The role of ceramid...
The role of ceramide in receptor- and stress-induced apoptosis studied in acidic ceramidase-deficient Farber disease cells
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- Burek, C. J. (author)
- University of Münster, Germany
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- Roth, J. (author)
- University of Münster, Germany
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- Koch, H. G. (author)
- University of Münster, Germany
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- Harzer, K. (author)
- University of Tübingen, Germany
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- Los, Marek Jan (author)
- Department of Immunology and Cell Biology, University of Münster, Germany
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- Schulze-Osthoff, Klaus (author)
- University of Münster, Germany
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(creator_code:org_t)
- 2001-10-19
- 2001
- English.
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In: Oncogene. - : Nature Publishing Group. - 0950-9232 .- 1476-5594. ; 20:45, s. 6493-6502
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Abstract
Subject headings
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- The activation of sphingomyelinases leading to the generation of ceramide has been implicated in various apoptotic pathways. However, the role of ceramide as an essential death mediator remains highly controversial. In the present study, we investigated the functional relevance of ceramide in a genetic model by using primary cells from a Farber disease patient. These cells accumulate ceramide as the result of an inherited deficiency of acidic ceramidase. We demonstrate that Farber disease lymphocytes and fibroblasts underwent apoptosis induced by various stress stimuli, including staurosporine, anticancer drugs and gamma -irradiation, equally as normal control cells. In addition, caspase activation by these proapoptotic agents occurred rather similarly in Farber disease and control fibroblasts. Interestingly, Farber disease lymphoid cells underwent apoptosis induced by the CD95 death receptor more rapidly than control cells. Our data therefore suggest that ceramide does not play an essential role as a second messenger in stress-induced apoptosis. However, in accordance with a role in lipid-rich microdomains, ceramide by altering membrane composition may function as an amplifier in CD95-mediated apoptosis.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- absence
- activation
- Apoptosis
- caspase-3
- cd95
- ceramidase
- Ceramide
- death
- Farber disease
- fas
- gd3 ganglioside
- Mice
- niemann-pick-disease
- Sphingomyelinase
Publication and Content Type
- ref (subject category)
- art (subject category)
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