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Il-2 Gene-Expressio...
Il-2 Gene-Expression and Nf-Kappa-B Activation Through Cd28 Requires Reactive Oxygen Production by 5-Lipoxygenase
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- Los, Marek Jan (author)
- Institute of Biochemistry, Albert-Ludwigs-University, Freiburg, Germany
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- Schenk, H. (author)
- Institute of Biochemistry, Albert-Ludwigs-University, Freiburg, Germany
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- Hexel, K. (author)
- Institute of Biochemistry, Albert-Ludwigs-University, Freiburg, Germany
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- Baeuerle, Pa (author)
- Institute of Biochemistry, Albert-Ludwigs-University, Freiburg, Germany
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- Droge, W. (author)
- Institute of Biochemistry, Albert-Ludwigs-University, Freiburg, Germany
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- Schulze-Osthoff, Klaus (author)
- Institute of Biochemistry, Albert-Ludwigs-University, Freiburg, Germany
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(creator_code:org_t)
- 1995
- 1995
- English.
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In: EMBO Journal. - 0261-4189 .- 1460-2075. ; 14:15, s. 3731-3740
- Related links:
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Activation of the CD28 surface receptor provides a major costimulatory signal for T cell activation resulting in enhanced production of interleukin-2 (IL-2) and cell proliferation. In primary T lymphocytes we show that CD28 ligation leads to the rapid intracellular formation of reactive oxygen intermediates (ROIs) which are required for CD28-mediated activation of the NF-kappa B/CD28-responsive complex and IL-2 expression. Delineation of the CD28 signaling cascade was found to involve protein tyrosine kinase activity, followed by the activation of phospholipase Az and 5-lipoxygenase. Our data suggest that lipoxygenase metabolites activate ROI formation which then induce IL-2 expression via NF-KB activation. These findings should be useful for therapeutic strategies and the development of immunosuppressants targeting the CD28 costimulatory pathway.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- cd28
- costimulation
- human monocytes
- il-2
- lymphocytes-t
- mediated suppression
- molecule cd28
- nf-kappa-b
- nuclear factor
- pathway
- receptor
- roi
- t-cell activation
- transcription
- tumor-necrosis-factor
Publication and Content Type
- ref (subject category)
- art (subject category)
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