Ratiometric temperature sensing with semiconducting polymer dots

Ye, FangmaoDepartment of Chemistry, University of Washington, Seattle, USA (author)

This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dye—Rhodamine B (RhB), whose emission intensity decreases with increasing temperature—within the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting and amplified energy transfer capability of Pdots. More importantly, the Pdot-RhB nanoparticle showed ratiometric temperature sensing under a single wavelength excitation and has a linear temperature sensing range that matches well with the physiologically relevant temperatures. We employed Pdot-RhB for measuring intracellular temperatures in a live-cell imaging mode. The exceptional brightness of Pdot-RhB allows this nanoscale temperature sensor to be used also as a fluorescent probe for cellular imaging.

Wu, ChangfengDepartment of Chemistry, University of Washington, Seattle, USA (author)
Jin, YuhuiDepartment of Chemistry, University of Washington, Seattle, USA (author)
Chan, Yang-HsiangDepartment of Chemistry, University of Washington, Seattle, USA (author)
Zhang, XuanjunDepartment of Chemistry, University of Washington, Seattle, USA(Swepub:liu)xuazh91 (author)
Chiu, Daniel TDepartment of Chemistry, University of Washington, Seattle, USA (author)
Department of Chemistry, University of Washington, Seattle, USA (creator_code:org_t)

In:Journal of the American Chemical Society: American Chemical Society (ACS)133:21, s. 8146-81490002-78631520-5126

By the author/editor: Ye, Fangmao; Wu, Changfeng; Jin, Yuhui; Chan, Yang-Hsian ...; Zhang, Xuanjun; Chiu, Daniel T

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