Design and testing of a packaged microfluidic cell for the multiplexed electrochemical detection of cancer markers

• We present the rapid prototyping of electrochemical sensor arrays integrated to microfluidicstowards the fabrication of integrated microsystems prototypes for point-of-carediagnostics. Rapid prototyping of microfluidics was realised by high-precision milling ofpolycarbonate sheets, which offers flexibility and rapid turnover of the desired designs. Onthe other hand, the electrochemical sensor arrays were fabricated using standard photolithographicand metal (gold and silver) deposition technology in order to realise threeelectrodecells comprising gold counter and working electrodes as well as silver referenceelectrode. The integration of fluidic chips and electrode arrays was realised via a lasermachineddouble-sided adhesive gasket that allowed creating the microchannels necessaryfor sample and reagent delivery. We focused our attention on the reproducibility of theelectrode array preparation for the multiplexed detection of tumour markers such ascarcinoembryonic antigen and prostate-specific antigen as well as genetic breast cancermarkers such as estrogen receptor-a, plasminogen activator urokinase receptor, epidermalgrowth factor receptor and erythroblastic leukemia viral oncogene homolog 2. We showedthat by carefully controlling the electrode surface pre-treatment and derivatisation viathiolated antibodies or short DNA probes that the detection of several key health parameterson a single chip was achievable with excellent reproducibility and high sensitivity.

Subject headings

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Keyword

Electrode arrays

Electrochemical biosensor

Microsystem

Tumour markers

Publication and Content Type

ref (subject category)

art (subject category)