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Ribonucleotides are not channeled into DNA in permeabilized mammalian cells
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- Spyrou, Giannis (author)
- Karolinska institutet, Stockholm, Sweden
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- Reichard, Peter (author)
- Karolinska institutet, Stockholm, Sweden
- (creator_code:org_t)
- Elsevier, 1983
- 1983
- English.
- In: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier. - 0006-291X .- 1090-2104. ; 115:3, s. 1022-1026
- Journal article (peer-reviewed)
Abstract
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- Reddy and Pardee earlier reported that permeabilized CHEF/18 cells (J. Biol. Chem 257, 12526) or soluble extracts from such cells (Cell 32, 443) under the proper conditions preferentially incorporated a labeled ribonucleotide (CDP) into DNA without dilution by an excess of non-labeled dCTP, suggesting channeling of ribonucleotides into DNA by a multiprotein complex. Here we present results suggesting that in these experiments CDP was incorporated into RNA and not into DNA.
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