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Overexpression of enzymatically active human cytosolic and mitochondrial thioredoxin reductase in HEK-293 cells : Effect on cell growth and differentiation

Nalvarte, Ivan (author)
Karolinska Institutet
Damdimopoulos, Anastasios E (author)
Karolinska Institutet
Nystöm, Christina (author)
Department of Laboratory Medicine, Division of Pathology, F46, Karolinska Institutet, Karolinska University Hospital Huddinge, Sweden
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Nordman, Tomas (author)
Department of Laboratory Medicine, Division of Pathology, F46, Karolinska Institutet, Karolinska University Hospital Huddinge, Sweden
Miranda-Vizuete, Antonio (author)
Karolinska Institutet, Huddinge, Sweden
Olsson, Jerker M. (author)
Department of Laboratory Medicine, Division of Pathology, F46, Karolinska Institutet, Karolinska University Hospital Huddinge, Sweden
Eriksson, Lennart (author)
Karolinska Institutet
Björnstedt, Mikael (author)
Karolinska Institutet
Arnér, Elias S.J. (author)
Karolinska Institutet
Spyrou, Giannis (author)
Department of Biosciences at Novum, Center for Biotechnology, Karolinska Institutet, Huddinge, Sweden / Institute of Biomedical Research, Academy of Athens, Greece
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 (creator_code:org_t)
American Society for Biochemistry and Molecular Biology, 2004
2004
English.
In: Journal of Biological Chemistry. - : American Society for Biochemistry and Molecular Biology. - 0021-9258 .- 1083-351X. ; 279:52, s. 54510-54517
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  • Journal article (peer-reviewed)
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  • The mammalian thioredoxin reductases (TrxR) are selenoproteins containing a catalytically active selenocysteine residue (Sec) and are important enzymes in cellular redox control. The cotranslational incorporation of Sec, necessary for activity, is governed by a stem-loop structure in the 3'-untranslated region of the mRNA and demands adequate selenium availability. The complicated translation machinery required for Sec incorporation is a major obstacle in isolating mammalian cell lines stably overexpressing selenoproteins. In this work we report on the development and characterization of stably transfected human embryonic kidney 293 cells that overexpress enzymatically active selenocysteine-containing cytosolic TrxR1 or mitochondrial TrxR2. We demonstrate that the overexpression of selenium-containing TrxR1 results in lower expression and activity of the endogenous selenoprotein glutathione peroxidase and that the activity of overexpressed TrxRs, rather than the protein amount, can be increased by selenium supplementation in the cell growth media. We also found that the TrxR-overexpressing cells grew slower over a wide range of selenium concentrations, which was an effect apparently not related to increased apoptosis nor to fatally altered intracellular levels of reactive oxygen species. Most surprisingly, the TrxR1- or TrxR2-overexpressing cells also induced novel expression of the epithelial markers CK18, CK-Cam5.2, and BerEP4, suggestive of a stimulation of cellular differentiation.

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